Merging Science & Nature 

Improving the health of your skin from the inside out and the outside in.    
MORE VIBRANT SKIN FROM 

Naturally-derived,98.6%  DAY ONE. plant-based ingredients

Merging Science & Nature Improving the health of your skin 

from the inside out and the outside in. Inspired by nature. Scientifically proven.
Four breakthrough products. One simple and remarkably effective skin-loving

solution. With a   24/7 approach to skin nutrition that merges   science and nature,

Alavida revives the radiance your skin once had. The beauty is effortless.

The results, visible.

 THE ALAVIDA SYSTEM

 Four innovative products that work independently.   When used

together, the results are visible, long-lasting, and life-changing. A beautiful merging of nature and science.

98.6% naturally-derived, plant-based ingredients. 

Reduces the appearance of fine lines. Brightens complexion for a regenerated, youthful glow naturally-derived, plant-based ingredients and wrinkles. Improves the appearance of

even skin tone. Hydrates 24/7 Improves the appearance of firm skin. 

Daily Refresh Facial Nectar

This two-in-one moisturizer product is for daytime use, when

your skin is exposed to the elements. Its lightweight consistency

is perfect for your pre-makeup routine.

Nearly 90% of subjects saw significant, overall improvement in appearance after

Nightly Restore Facial Créme. This two-in-one moisturizer product is for nighttime use, when

your body naturally replenishes itself. An ideal complement to the

Daily Refresh Facial Nectar. 3 weeks*

Alavida Revive Eye Cream

This copper peptide-infused eye cream uses a blend of powerful ingredients, providing unparalleled benefits to help brighten complexion, improve the appearance of even skin tone, and

support the appearance of firm skin.

Alavida Patch

Alavida harnesses and balances your body’s natural restorative energy to improve the healthy and radiant appearance of your skin. Also for night   time use.

MERGING SCIENCE & NATURE

Millions of people around the world struggle with the visible signs of

aging, especially around their eyes. Stress, genetics, environmental

factors, poor nutrition, and long workdays all contribute to signs of

aging around your eyes.

We all want to look and feel our best and   our eyes are one area that

we want to sparkle and appear youthful for as long as we can.

That’s where LifeWave can help, by offering a new revolutionary eye

cream that merges science and nature to help   you revive your eyes for

a more youthful-looking appearance.

At LifeWave, we know you want to age confidently and beautifully! We

believe that your eyes should be as bright and radiant as you are on

the inside. We get   it. That’s why we created Alavida Revive Eye Cream.

Merging science and nature, the entire Alavida Regenerating

System combines a powerful suite of ingredients to help brighten complexion, improve

the appearance of even skin tone, and support the appearance of firm skin.

THE SCIENCE INSIDE.

Developed to work for all skin types, Alavida products apply

complex science to ingredients proven to have a positive impact

on health and complexion. Yet, the solutions go beyond that.

The formulations and innovative delivery methods have created

breakthrough results.   

100% of subjects saw an immediate, significant improvement in skin hydration*

Source: Clinical   Evaluation of the Efficacy of a Skin Care  Regimen to Improve Skin Conditions

GHK-CU COPPER PEPTIDE

This copper peptide, which is naturally occurring in the body, is known to support

collagen and elastin. Collagen and   elastin are essential proteins that keep skin

looking firm and youthful, and the   addition of GHK-Cu to the Alavida Revive Eye

Cream may help to replenish these proteins and reduce the appearance of fine

lines and   wrinkles.

MICROALGAE OIL (TRIOLEIN)

A natural form of algae that is rich in Omega 9

A superior increase in skin moisturization and a greatly improved protective barrier.

EVENING PRIMROSE OIL

Contains Gamma-Linolenic Acid (GLA) and other polyunsaturated fatty acids.

Deep moisturizing of dry, itchy, or chapped skin.

MILK PEPTIDES

Naturally sourced from milk in its bio-activated and stabilized form

Boosts collagen and increases the production of hyaluronic acid to reduce the

appearance of fine lines and   wrinkles and improve the appearance of skin firmness.**

MORINGA  BUTTER

Naturally derived from the   seed oil of India’s Moringa oleifera “miracle tree”

Rejuvenate your skin by reinventing the way you treat it.

Everyone has the right to feel beautiful inside and out, with a bright   outlook

on life and an equally vibrant glow. Find out how you can make Alavida a

part of your life by contacting   your LifeWave Brand Partner or learning more

online. Start today.

LifeWave, Inc.

9775 Businesspark Ave,

San Diego, CA, USA

Tel/Fax: +1(858) 459-9876

customerservice@lifewave.com

LifeWave Europe, Ltd.

Raheen Ind. Est., Athenry, Co.

Galway, IRL

Tel: +353 (0) 91 874 600

customerserviceeu@lifewave.com

W W W. L I F E WAV E . C O M

GHK-Cu Copper Peptide:

Dr. Loren Pickart discovered GHK and many of its biological effects with his original thesis is titled:

"A TriPeptide From Human Serum”

(June 1973)

GHK-Cu Copper Peptide activates Stem Cells for full body repair and regeneration!

GHK-Cu Copper Peptide is proven to ACTIVATE your stem cells, PRODUCE more stem cells, and RESET your stem cells to a younger healthier state.

Why is that important? Stem cells have the unique ability to change into any cell your body needs for repair and regeneration, just like when you were younger. 

Our cells are constantly wearing out, forcing our body to work around the clock to make new and healthy cells. Your activated younger stem cells turn those old cells young and healthy again! In fact, within 24 hours, GHK-Cu Copper Peptide resets 4,000 genes to a younger, healthier state.  As we age, we produce less and less stem cells, slowing down our body's ability to repair itself. By age 35 we have lost 50% of our stem cell activity. By age 65 we have lost almost all our stem cell activity. When we activate our body with these younger pluripotent stem cells, they have the unique ability to turn into whatever cell the body needs for repair.

Your new stem cell army is now activated! Even if you don’t immediately feel the effects, rest assured your army of stem cells is now hard at work repairing your most critical issues first before moving on to something you do feel.

•GHK tightens loose skin and thicken older skin

•GHK reduces fine lines and depth of wrinkles

•GHK increases hair growth and thickness

•GHK accelerates wound healing

•GHK tops the list for chronic pulmonary issues

•GHK restores healthy function in lung cells

•GHK protects lung cells from damage

•GHK tops the list to combat Fast Growing COLON cells

•GHK resets the PROGRAMMED CELL DEATH for aging cells

•GHK inhibites NFKB p65 which promotes fast growing cells

•GHK repaires damaged cells (47 genes up, 5 genes down) 

•GHK resets 84 genes to inhibit fast growing cells

•GHK resets human genes important to proper neuron function with 408 UP and 230 DOWN • GHK induces strong Anti-Stress & Anti-Anger

•GHK induces Anti-Discomfort Activity

•GHK is being used for damaged spinal cords

•GHK increases SOD1

•GHK suppresses NFKB, a primary cause of aging

•GHK completely blocks oxidation

ACTIVATES STEM CELLS

• GHK causes adult Stem Cells to start producing again • GHK produces more Stem Cells and resets Stem Cells to a younger, healthier state

  each sleeve contains 30 patches

Retail     Price

(One Time Purchase) $149.95 Commissions Paid $50.00 (One Time Purchase) Volume

77 PV

$99.95 (Monthly  Autoship) $20.00 (Every Month) Volume 43 PV

2. PRODUCTION INTRODUCTION BONUS (PE)

*The Premium Pack $1,750, PIB $405, 745 BV is divided into 3 increments.

1st Month 525 BV, 2nd Month 110 BV, and 3rd Month 110 BV. 40 Sleeves

** 1 Sleeve of X39@ or X49TM = 2 Sleeves of any other Supportive Patches

*The Advanced Pack $535, PIB $75, 300 BV,   12 sleeves **1 Sleeve of X39@ or X49TM = 2 Sleeves of any other Supportive Patches

*The Core Pack $295, PIB $35, 180 BV, 6 sleeves **1 Sleeve of X39@ or X49TM = 2 Sleeves of any other Supportive Patches

Active = Pack + 55 PV pts / 1 Sleeve per month autoship

Qualified = Enroll 1 Person on Left & Enroll 1 Person on Right

is active on   55 PV pts or more a month active on 55 a month active on 55 a month

PV pts or 1 = 1 cycle $50

Multiple Cycling = More   Money

1 330 BV pts 660 BV pts

Single Sleeve BV Volume = X39 or X49 77 BV pts I Single Supportive Patch = 55 BV Pts

PV = Personal Volume I BV = Business Volume I BP = Brand Partner

Move Up In Rank

"Become a Manager then make Managers"

Minimum   Lifetime PV of 300 (Advanced pack or higher purchase)

Be active   on 110 PV pts per month autoship

2 personally sponsored Active BPS with a minimum Lifetime PV of 1 80 (Core pack or higher purchase) on your Left  leg

Teach others to do the same! MAXIMUM WEEKLY

BINARY COMMISSION $100

Binary Qualified BP $1,500

Manager

Director $2,500

Senior Director $3,500 $7,500

Executive Director

Presidential Director $12,500

Senior Presidential Director $25,000

PAID-AS RANK   
4. UNLIMITED MATCHINC BONUSES Based on Your Binary Commission

1st level 25% Match Active 110 PV Qualifications is paid as Manager or above

Level 2 20% Match Active 110 PV Paid as Manager or above minimum 6 cycles in paid commissions weekly 

Level 3 20% Match Active 110 PV 3 personally sponsored Active BPs with a minimum Lifetime PV of 180 on each leg and 1 personally sponsored PB earning Level 2 matching on each leg minimum 10 cycles in paid commissions weekly

DISTRIBUTOR RANK QUALIFICATIONS

MANAGER

• Have a minimum Lifetime PV of 300 (Advanced pack or higher purchase)

• Generate   a minimum of 110 PV per month

• Have 2   personally sponsored Active BPS with a minimum Lifetime PV of 180 (Core pack   or higher purchase) on each leg

DIRECTOR

• Have a   minimum Lifetime PV of 300 (Advanced pack or higher purchase)

• Generate   a minimum of 110 PV per month

• Have 3   personally sponsored Active BPS with a minimum Lifetime PV of 180 (Core pack   or higher purchase) on each leg

• Have I   personally sponsored Brand Partner who achieves Manager status on each leg

SENIOR DIRECTOR

• Must be   Director status qualified

• Have 2   personally sponsored Brand Partner who achieves Director status

• Accumulate   10,000 BV through line of sponsorship in 31 day period

EXECUTIVE DIRECTOR

• Must be  Senior Director status qualified

• Accumulate   50,000 BV through line of sponsorship in 31 day period

PRESIDENTIAL DIRECTOR

• Must be   Senior Director status qualified

• Accumulate   100,000 BV through line of sponsorship in 31 day period

SENIOR PRESIDENTIAL DIRECTOR

• Must be   Senior Director status qualified

• Accumulate   200,000 BV through line of sponsorship in 31 day period

  • Clinically shown to increase length of sleep by 66%

  • Enhances the quality of sleep

  • Patented, proprietary form of phototherapy

  • No drugs, chemicals or stimulants

A Healthy Sleep-Aid Alternative

 Free of drugs, chemicals or stimulants, Silent Nights is clinically proven to improve quality and length of sleep without causing that groggy feeling the next day. You’ll wake up feeling well rested, more energetic and better prepared to make the most out of life.

What Is Phototherapy?

The science of phototherapy, which has been around for about 100 years, uses light to improve the health of the body. And modern forms of phototherapy such as Low Level Laser Therapy, which helps reduce wrinkles in the skin, are very well understood scientifically.

But this idea is nothing new. As far back as two thousand years ago, the ancient Greeks had a center for studying the effects of different colored lights on human health. Even the ancient Egyptians, who promoted health by focusing sunlight through colored glass on certain areas of the body, understood this concept.

How Our Phototherapy Patches Work

Your body emits heat in the form of infrared light. Our patches are designed to trap this infrared light when placed on the body, which causes them to reflect particular wavelengths of light. (see Usage Tab for placement instructions). This process stimulates specific points on the skin that signal the body to produce health benefits unique to each LifeWave patch.
What Makes one LifeWave Patch Different than Another?

Each patch is exclusively designed to reflect particular wavelengths of light that stimulate specific points on the skin. This enables each patch to provide unique health benefits. No drugs or chemicals enter your body.

How Does This Relate to Healthy Sleep?

Silent Nights reflects particular wavelengths of light, which stimulate specific points on the skin that trigger the production of melatonin in the body.

Silent Nights Study Results

Silent Nights is clinically proven to increase length of sleep by 66 percent, and since its release has helped people all over the world achieve better rest. Subsequent to its release, a pilot study conducted by Dr. Norm Shealy concluded, “The safety and results obtained in the study of Silent Nights suggests that these patches may be one of the preferred potential approaches to significant improvement in sleep.”

Our influencer marketing services are designed to help businesses reach their target audience through trusted influencers. We identify and partner with influencers who align with your brand values and create effective influencer campaigns that drive engagement and conversions.

 X39™ is a STEM   CELL ACTIVATION patch designed to activate stem cell production by   elevating GHK-Cu peptide. It resets   4000+ genes to a younger state, helps repair collagen in tissues, activates   stem cell production, encourages apoptosis of cancer cells and discourages   metastatic cancer cells. Using X39™ people have better sleep, less pain and   inflammation, and faster wound healing. 20 year patent! 

X49™ elevates natural levels of AHK-Cu, a copper   peptide that activates vascular endothelial cells to support healthy blood   flow. It increases energy and recovery after exercise, improves   cardiovascular function and focus, reduces belly fat and increases muscle   tone, strength, stamina, lean body mass and bone density. Assists with osteoporosis. Provides support   for brain issues. 

Alavida is for skin & body regeneration via   elevation of the epithalamin peptide known to increase organ function, reduce oxidative stress, increase   antioxidants, stimulate frontal lobes and activate the pineal gland, increase   mental clarity and focus, and help with sleep. Second most powerful patch after X39™. The Alavida   Regenerating Trio comes with 2 sleeves of Alavida (30 patches in each), 1 daily nectar   and 1 night crème, enough for two   months. 98.6% plant derived   ingredients. 

AEON is an anti-inflammatory, anti-stress, happy   patch. It raises Antineoplastons,   balances & calms the brain, reduces cortisol, reduces c-reactive proteins, & elevates DHEA. AEON  is equivalent to eating 30 cups of Royal Jelly a day. Phenyl Acetyl Glutamine is a substance that   is elevated in the Liver. It is part of the primitive immune system and it’s   not an antioxidant but induces   antioxidant activity in the cells. It   upregulates production of superoxide dismutase or SOD which causes a broad   spectrum reduction of inflammatory markers such as c-reactive protein,   inflammatory cytokines, homocysteine, lipid peroxide and fibrinogen. Used with X39™ the effect is enhanced since inflammation destroys stem cells. 

Carnosine is the heart and brain patch . It is known   as the first aid patch and is great for burns, lumps, bumps, bruises, lacerations and clots. It is great for tissue repair, healing, and   longevity. It increases cellular   communication. Carnosine is stored in the heart, brain, and skeletal muscles. It is beneficial for all heart and blood sugar issues. It protects against cardiovascular disease,   beneficial for diabetes and preserves and supports cognitive function and neurological issues. It helps build skeletal muscle mass and   enhances muscle strength by 125%. It   helps to protect the telomeres and aids in wound healing. It pulls aluminum from the brain. Most people are deficient in Carnosine. 

Glutathione   is the MASTER antioxidant, detoxifier,   and immune booster. It is a vital intracellular tripeptide   molecule that plays a central role in cellular physiologic functions. It has the ability to minimize oxidative   stress. The Glutathione patch   increases Glutathione by 300% in 24 hours where Glutathione infusions only   stay in the body for a short period of time. It is an anti-viral and assists   with infections. 

SP6   Complete controls   appetite & suppresses cravings, balances hormones and increases organ   function. Helps improve digestive   function. Supports the endocrine   system and balances the hypothalamus which regulates the autonomic nervous   system. One study noted a significant improvement in the functioning of several major organs, including the   hypothalamus, thyroid, adrenal glands, kidneys, liver, pancreas, &   intestines. 

Energy   Enhancer increases   energy and endurance, reduces fatigue and muscle soreness. It can be used on acupuncture points bilaterally for nausea, reflux, to improve   digestion, strengthen and tonify organs, drain dampness and phlegm, and   increase Qi Bioenergy in organs. Similar to RED laser. Great for helping to remove congestion   from the Lungs and improve breathing. A two patch system. 

ICEWAVE decreases pain. It reduces or eliminates all types of pain   such as pulled nerves and muscles, osteoarthritis, fibromyalgia, stiffness, migraines, and headaches. It sedates excess energy in the connective   tissue and reduces inflammation which causes pain, and has a calming   effect in the pain area. It is similar to BLUE laser. 

Silent   Nights improves   sleep. It regulates sleep through   serotonin production which modulates melatonin. Also beneficial for oxidative stress as   melatonin is an antioxidant and cancer protective. Great for calming ASD children. Melatonin is a protectorate of   telomeres. Studies showed significant enhancement on the   functioning of the cardiac ventricles and thyroid glands. 

A blend of natural herbs and essential oils   embedded into a proprietary energized and structured water allowing for rapid results. SHINE encourages feelings of   wellness & energy with an invigorating aroma that awakens the senses in the morning. DREAM   creates a zen-like environment and promotes feelings of peace, rest and   relaxation at bedtime. 

CALL FOR A FREE 30 MINUTE CONSULTATION 

Lauren Engel

(254) 368-7778

Go to www.STARTX39NOW.com for the Science, Patents, 

Testimonials, Clinical Studies, and Patch Placement   Aromatherapy Mist is no longer available!

Our influencer marketing services are designed to help businesses reach their target audience through trusted influencers. We identify and partner with influencers who align with your brand values and create effective influencer campaigns that drive engagement and conversions.

  • Safe and natural pain relief

  • Can be used for whole body and local pain

  • Fast-acting and non-addictive

  • Convenient and easy to use

  • Patented, proprietary form of phototherapy

  • No drugs, chemicals or stimulants

Chronic pain, alone, affects 1.5 billion people around the world, leading to billions of dollars in health care costs and lost work productivity each year.1 IceWave is a safe, powerful and affordable solution for all levels of pain.

Using the healing properties of light, IceWave is designed to provide fast relief at the source of discomfort. If you have knee pain, for instance, you can place the patches around that area for quick relief. If you have pain throughout your body, there are different placement options that produce equally effective results (see the Usage Tab for easy instructions).

This non-drug, non-addictive approach sets IceWave apart from all other pain relief products on the market. Particularly in contrast to mainstream pharmaceutical painkillers, which are known to have harmful side effects, including widespread opioid addiction plaguing many communities in the U.S. today.

But the problem isn’t just in America. New research on prescription drug use in Denmark, Germany, Spain, Sweden and the United Kingdom estimates the lifetime rate of opioid use at nearly 14 percent. This is compared to just 5 percent in the previous year.2

1) David Borsook, M.D., Ph.D, a leading pain expert at Massachusetts General Hospital in the U.S. 

2) RTI International, 2015

What Is Phototherapy?
The science of phototherapy, which has been around for about 100 years, uses light to improve the health of the body. And modern forms of phototherapy such as Low Level Laser Therapy, which helps reduce wrinkles and treat other more serious skin conditions, are very well understood scientifically.

But this idea is nothing new. As far back as two thousand years ago, the ancient Greeks had a center for studying the effects of different colored lights on the body. Even the ancient Egyptians, who promoted health by focusing sunlight through colored glass on certain areas of the body, understood this concept. 

Your body emits heat in the form of infrared light. Our patches are designed to trap this infrared light when placed on the body, which causes them to reflect particular wavelengths of light. (see Usage Tab for placement instructions). This process stimulates specific points on the skin that signal the body to produce health benefits unique to each LifeWave patch.

What Makes one LifeWave Patch Different than Another?

Each patch is exclusively designed to reflect particular wavelengths of light that stimulate specific points on the skin. This enables each patch to provide unique health benefits (e.g. pain relief, increased energy, etc.). No drugs or chemicals enter your body.

How Does This Relate to Pain?

Pain is caused by a change in tissue conductivity. By reflecting particular wavelengths of light, IceWave patches improve tissue 

conductivity. This means they use the body’s natural energy flow (or bioelectricity) to reduce pain. IceWave patches have also been shown to reduce inflammation using this same method.

  • Glutathione is the body’s master antioxidant

  • Supports the immune system

  • Improves overall health

  • Patented, proprietary form of phototherapy

  • No drugs, chemicals or stimulants 

A Strong Immune System is Your Greatest Defense

Glutathione is the body’s master antioxidant and primary antioxidant for detoxification. It’s found in nearly every human cell, and medical experts say that lifespan is directly equivalent to the amount of Glutathione in your body. With its ability to protect and detoxify, it also provides crucial immune system support. It’s also worth noting that glutathione is incredibly well researched. In fact, thousands of clinical studies have been conducted on this powerful antioxidant.

As the primary defense against invaders from outside and inside the body, the importance of the immune system cannot be overstated. The good news is this patch is clinically proven to keep yours strong. No other single product can support your immune system like Y-Age Glutathione, and this patch does it all with no drugs or chemicals.

What Is Phototherapy?

The science of phototherapy, which has been around for about 100 years, uses light to improve the health of the body. And modern forms of phototherapy such as Low Level Laser Therapy, which helps reduce wrinkles in the skin, are very well understood scientifically.

But this idea is nothing new. As far back as two thousand years ago, the ancient Greeks had a center for studying the effects of different colored lights on human health. Even the ancient Egyptians, who promoted health by focusing sunlight through colored glass on certain areas of the body, understood this concept.

How Our Phototherapy Patches Work

Your body emits heat in the form of infrared light. Our patches are designed to trap this infrared light when placed on the body, which causes them to reflect particular wavelengths of light. (see Usage Tab for placement instructions). This process stimulates specific points on the skin that signal the body to produce health benefits unique to each LifeWave patch.

What Makes one LifeWave Patch Different than Another?

Each patch is exclusively designed to reflect particular wavelengths of light that stimulate specific points on the skin. This enables each patch to provide unique health benefits. No drugs or chemicals enter your body.

How Does This Relate to Immune System Support?

Y-Age Glutathione reflects particular wavelengths of light, which stimulate specific points on the skin that increase production of glutathione in the body. This helps decrease inflammation and increase antioxidant production, all of which leads to a stronger immune system 

  •  Clinically proven to increase energy and endurance

  •  Supports a physical fitness routine

  •  Convenient and easy to use

  •  Fast results

  •  Patented, proprietary form of phototherapy

  •  No drugs, chemicals or stimulants

Before starting LifeWave in 2004, our Founder and CEO (David Schmidt) was already compelled to answer one persisting question: how can people sustain energy without using drugs, stimulants, or caffeine? The answer led to the development of Energy Enhancer, the first patch we ever developed and the foundation of our patented phototherapy technology.

Shortly after Energy Enhancer was released, David was introduced to renowned women’s swimming coach, Richard Quick, of Stanford University. Just three weeks after providing the patches to his team, six of its eight members broke their personal lifetime records. Stanford Team members were then spotted wearing the patches during the Olympic Swimming trials, propelling LifeWave into the national media spotlight. As a result, over 1000 people came forward to become LifeWave Distributors.

Since that time, Energy Enhancer has helped people around the globe go further, run faster, climb higher and embrace each day with renewed stamina and vigor. A safe more effective alternative to other energy products, Energy Enhancer will make you forget you ever hit that late-afternoon wall again.

What Is Phototherapy?

The science of phototherapy, which has been around for about 100 years, uses light to improve the health of the body. And modern forms of phototherapy such as Low Level Laser Therapy, which helps reduce wrinkles in the skin, are very well understood scientifically.

But this idea is nothing new. As far back as two thousand years ago, the ancient Greeks had a center for studying the effects of different colored lights on the body. Even the ancient Egyptians, who promoted health by focusing sunlight through colored glass on certain areas of the body, understood this concept.

Your body emits heat in the form of infrared light. Our patches are designed to trap this infrared light when placed on the body, which causes them to reflect particular wavelengths of light. (see Usage Tab for placement instructions). This process stimulates specific points on the skin that signal the body to produce health benefits unique to each LifeWave patch.

How Does This Relate to Increasing Energy?

Energy Enhancer patches reflect particular wavelengths of light, which stimulate specific points on the skin that increase energy production in the cells (beta-oxidation). Because beta-oxidation produces more than twice the energy of carbohydrate burning, this is the most effective and natural way to increase overall energy.

 Clinically proven to reduce stress in the body

 Promotes relaxation 

 Patented, proprietary form of phototherapy

 No drugs, chemicals or stimulants 

Stress Relief You Can Feel Good About

Nobody is immune to stress, but when used as part of a healthy lifestyle, Y-Age Aeon delivers powerful relief without a trip to the doctor or pharmacy. As a result, you’ll experience a greater quality of life, with no negative side effects.

What Is Phototherapy?

The science of phototherapy, which has been around for about 100 years, uses light to improve the health of the body. And modern forms of phototherapy such as Low Level Laser Therapy, which helps reduce wrinkles in the skin, are very well understood scientifically.

But this idea is nothing new. As far back as two thousand years ago, the ancient Greeks had a center for studying the effects of different colored lights on human health. Even the ancient Egyptians, who promoted health by focusing sunlight through colored glass on certain areas of the body, understood this concept.

How Our Phototherapy Patches Work

Your body emits heat in the form of infrared light. Our patches are designed to trap this infrared light when placed on the body, which causes them to reflect particular wavelengths of light. (see Usage Tab for placement instructions). This process stimulates specific points on the skin that signal the body to produce health benefits unique to each LifeWave patch.

  
What Makes one LifeWave Patch Different than Another?

Each patch is exclusively designed to reflect particular wavelengths of light that stimulate specific points on the skin. This enables each patch to provide unique health benefits (e.g. pain relief, increased energy, etc.). No drugs or chemicals enter your body.

How Does This Relate to Stress Relief?

Y-Age Aeon reflects particular wavelengths of light, which stimulate specific points on the skin that decrease inflammation and increase antioxidant production in the body.

WHAT IS THE NAME OF THE PRODUCT(S)? 

Introducing the Alavida Regenerating System, featuring four exceptional components: Alavida Patches, Alavida Nightly Restore Facial Crème, Alavida Daily Refresh Facial Nectar, and Alavida Revive Eye Cream! Product availability and offerings may vary based on the market.

WHAT IS PHOTOTHERAPY? 

The science of phototherapy, which has been around for about 100 years, uses light to improve the health of the body. As far back as 2,000 years ago, the ancient Greeks had a center for studying the effects of different colored lights on the body. Even the ancient Egyptians, who promoted health by focusing sunlight through colored glass on certain areas of the body, understood this concept. 

HOW DO LIFEWAVE PHOTOTHERAPY PATCHES WORK? 

Your body emits heat, including heat in the infrared spectrum. Our patches are designed to trap this infrared energy when placed on the body, which causes them to reflect it back to stimulate specific points on the skin that can promote a general state of health and healthy activity unique to each LifeWave patch. 

WHAT ARE THE INSTRUCTIONS FOR USE FOR ALAVIDA PRODUCTS? 

Alavida Patches: Apply one patch each night before going to bed. Place one patch on the body, using one of the locations shown below. Apply it to clean, dry skin before retiring. Patch may be worn for up to 12 hours before discarding. Use a new patch for each application.

Position 1 Position 2

Alavida Revive Eye Cream: Use around eye area in morning and if desired, at night.

Alavida Daily Refresh Facial Nectar: Apply onto clean skin of face and neck.

Alavida Nightly Restore Facial Crème: Apply onto clean skin of face and neck.

WOULD YOU RECOMMEND USING ALAVIDA ALONE OR AS PART OF A REGIMEN? 

1. Alavida Revive Eye Cream - This copper peptide-infused eye cream uses a blend of powerful ingredients, providing unparalleled benefits to help brighten complexion, improve the appearance of even skin tone, and support the appearance of firm skin. For best results use in conjunction with the Alavida Regenerating System. 

2. Nightly Restore Facial Crème - Alavida Nightly Restore Facial Crème supports overnight protection of your skin to reduce the appearance of fine lines and wrinkles, brighten complexion. It is an ideal complement to the Daily Refresh Facial Nectar.

3. Daily Refresh Facial Nectar - This two-in-one moisturizer supports protection of your skin, while improving radiance and appearance with daily application. Its lightweight consistency is perfect for your pre-makeup routine.

4. Alavida Patch - Also for nighttime use, with the Nightly Restore Facial Crème.

5. Combine it with LifeWave X39 - During the day.

CAN THEY BE USED BY CHILDREN AND/OR PREGNANT WOMEN?

We do not recommend the Alavida System for use by anyone under 18 years old, or by anyone who is pregnant and/or breastfeeding. 

WHAT ARE THE PRECAUTIONS?

Alavida Patches: Do not use if pregnant or nursing. Remove immediately if you feel discomfort or skin irritation. If irritation persists, consult your health professional. Keep well hydrated prior to using this product. Do not reuse patch once removed from the skin. For external use only. Do not ingest. Do not use on wounds or damaged skin. 

Alavida Eye Cream, Crème and Nectar: Avoid contact with eyes. If skin irritation occurs, please discontinue use. If irritation persists, consult your health professional.

HOW SHOULD I STORE ALAVIDA PRODUCTS?

Alavida Patches: Store in a cool, dark place, out of direct sunlight. Product should not be exposed to temperatures greater than 140°F/40°C and should be stored at room temperature.

Alavida Eye Cream, Crème and Nectar: Store in a cool, dark place.

HOW SHOULD I DISPOSE OF THESE PRODUCTS WHEN I’M DONE WITH THEM?

All Alavida containers and patches can be disposed of in general waste.

WHAT IS THE SHELF LIFE OF THE ALAVIDA SYSTEM?

The shelf life for Alavida Patches is 30 months.

The shelf life for Alavida Revive Eye Cream is 18 months.

The shelf life for Alavida Crème and Alavida Nectar is 36 months. 

CAN I MAKE MEDICAL CLAIMS ABOUT ANY OF THE PRODUCTS IN THE ALAVIDA SYSTEM?

Our products are not designed to treat specific conditions/diseases, nor do we create patch protocols designed to treat any specific condition/disease. 

MARKET AVAILABILITY

Where are Alavida products available?

Our products are available worldwide, depending on the market. To check availability in your country, please visit our website and select the appropriate country and language. 

PRICING  

BRAND PARTNER PRICING

Alavida Patches $69.95/€66 | 55BV

Alavida Revive Eye Cream $69.95/€66 | 42BV

Alavida Nightly Restore Facial Crème $79.95/€76 | 55BV

Alavida Daily Refresh Facial Nectar $59.95/€57 | 42BV

Alavida Regenerating Trio $149.95/€142 | 119BV

PREFERRED CUSTOMER   PRICING BONUS

Alavida Patches $69.95/€66 | 39BV $20 €19

Alavida Revive Eye Cream $69.95/€66 | 17BV $15 €14

Alavida Nightly Restore   Facial Crème $79.95/€76 | 21BV $20 €19

Alavida Daily Refresh Facial Nectar $59.95/€57 | 25BV $15 €15

Alavida Regenerating Trio $149.95/€142 | 51BV $40 €38

RETAIL   CUSTOMER PRICINGBONUS

Alavida Patches $79.95/€76 | 39BV $30 €29

Alavida Revive Eye Cream $89.95/€85 | 42BV $20 €19

Alavida Nightly Restore   Facial Crème $99.95/€95 | 55BV $20 €19

Alavida Daily Refresh   Facial Nectar $79.95/€76 | 42BV $20 €19

Alavida Regenerating Trio $199.95/€190 | 119BV $50 €48

ARE ALAVIDA PRODUCTS AVAILABLE AS PART OF ENROLLMENTS OR UPGRADE ORDERS? 

Yes, the Alavida Regenerating Trio and Alavida Patches are available as part of enrollments or upgrade orders. However, individual cosmetics like Alavida Crème, Nectar, and Eye Cream are not included in these offers.

ARE ALAVIDA PRODUCTS AVAILABLE AS PART OF MAINTENANCE PACKS? 

Yes.

CAN ALAVIDA PRODUCTS BE INCLUDED IN A MONTHLY SUBSCRIPTION ORDER?

Yes.

IS THE ALAVIDA REVIVE EYE CREAM PART OF THE ALAVIDA REGENERATING TRIO BOX?

Alavida Revive Eye Cream is not part of the Alavida Regenerating Trio box, and it can only be purchased as a standalone product.

WILL SHIPPING BE MORE EXPENSIVE IF I ORDER MULTIPLE PRODUCTS?

Shipping is calculated by weight so this will be determined by what is in your order.

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WHAT IS LIFEWAVE’S DISCLAIMER?

LifeWave products are for general wellness and intended only to maintain or encourage a general state of health or a healthy activity. The content provide by LifeWave is presented in summary form, is general in nature, and is provided for informational purposes only. Always consult with your physician or other qualified health care provider before embarking on a new health regimen, diet, or fitness program. LifeWave reserves the right to change or discontinue at any time information relating to LifeWave’s products.

HISTORICAL BREAKTHROUGH THAT ACTIVATES EXISTING STEM CELLS

 As we age, our stem cells decline in their ability to function and heal our bodies

LifeWave's stem cell activation patches are clinically proven to provide the body with a level of health and vitality that many have not experienced in years

 The secret? An exclusive, patented and proven phototherapy non-transdermal patch technology that is safe, non-invasive and easy to use

 LifeWave's  is the result of over 80 clinical studies and 20 years of research

When this wearable technology is applied to the body, the phototherapy patches are activated by your body's heat reflects specific wavelengths of light back toward the body, signaling the body to elevate the GHK-Cu copper peptide.

 GHK-Cu has been clinically proven to activate your body's own stem cells

LifeWave is the world's first and only company using this patent-pending approach to activate stem cells.

  
TYPICAL HEALTH BENEFITS

 Reduced pain and discomfort - Assists recovery from exercise

 Reduced   inflammation

Helps improve skin appearance

Better   sleep

Supports the natural   wound healing process

 Improved energy and vitality

Enhanced sports performance

Restores mental clarity

HOW QUICKLY DO PEOPLE EXPERIENCE X39'S BENEFITS?

The First Few Days - 4,000 Genes Begin to Reset

Within 24 hours the stem cell activation patch begins to reset over 4,000 genes to a younger state. "People using the stem cell patches will experience an immediate effect through the elevation of antioxidants and a decrease in inflamation and an increase in energy and joy." - Melinda H Connor, DD, November 2020

Within 1-3 Months - Physical, Mental & Emotional Changes Occur

Activating your stem cells leads to cellular repair, regeneration and age reversal bringing you to optimal health on all levels At 6 weeks your brain is balanced relieving symptoms of PTSD, anxiety and depression. At 2 months your heart/cardlovascular system is becoming younger. At 3 months your collagen is repairing. Even if you don't feel the effects, rest assured the new stem cells may be repairing an internal organ or tissue before repairing something you see or feel.

Within 3-6 Months - Active Stem Cell Repair

The plural potent stem cells turn into any cell your body needs for repair. Your stem cells are now actively creating deep healing in your body and repairing damage caused by the aging process.

Within 6-12 Months - Reverse Aging

In a recent study, 14 out of 15 people lowered their biological age by an average of 8 years after just 6 months using X39. li lt's not anti-aging, it's age reversing." -David Schmidt, LA Convention, June 2020

X39 R USAGE INSTRUCTIONS:

 Before using  watch the short 3-min video on the X39@technology

Complete the "Before" column on the  Health Benefits Tracker below

Apply one  Patch in the morning

Wear the  Patch for 12 Hours, REMOVE and DISCARD before bedtime

The next day, complete the "24 hours" column on the Health Benefits Tracker I or one of my team members will be happy to answer questions about this amazing technology  
Keep Tracking Your  benefits for 90 Days with the Health Benefits Tracker

PLACE ONE X39@ PATCH ON EITHER OF THESE TWO LOCATIONS

Apply to clean, dry skin in the morning oo

Patches may be worn for up to 12 hours before discarding

 Keep well-hydrated during your X39@ Experience

Warnings: Remove immediately if you feel excessive discomfort or if skin irritation occurs.

For external use only. Do not ingest. Do not use directly on open wounds or damaged skin. LIFEWRVE@

Ask your health professional before using if you have a health condition or have questions and concerns about your health. Do not use if pregnant or nursing.  

Introducing the X39® + X49™ Performance Bundle

LifeWave’s New Performance Bundle   includes 30 patches of X39® and 30 patches of X49™.

Both are to be   used at the same time, everyday, giving you one month’s supply.

The LifeWave X49™ patch elevates the peptide AHK-Cu that improves cognitive function

Clinically proven to enhance strength and stamina. Reduces muscle soreness and promotes recovery

from exercise.       

Support fat loss when   used with a healthy diet and exercise program.       

The LifeWave X39® patch elevates the peptide GHK-Cu for the activation of stem cells

Clinically proven to enhance overall health and vitality. Gives you a new level of   dynamic wellness

experience.

X39® and X49™ Synergy

By using LifeWave’s patented X49™ and X39® phototherapy patches together, you trigger a synergistic, multiplier eꢀect that   supports performance and shortens recovery time, allowing you to do more, better, faster.

Instructions

 X39® and X49™ also share the same patch placement, so it’s recommended to use 1 of each patch in 1 of each of these locations.

Apply the patches to clean,   dry skin in the morning. Patches may be worn for up to 12 hours before discarding. Keep well hydrated when using these products.

  Int J Mol Sci. 2018 Jul 7;19(7):1987. doi: 10.3390/ijms19071987

Loren Pickart 1, Anna Margolina 1,*

• Author information
• Article notes
• Copyright and License information

PMCID: PMC6073405  PMID: 29986520

The human peptide GHK (glycyl-l-histidyl-l-lysine) has multiple biological actions, all of which, according to our current knowledge, appear to be health positive. It stimulates blood vessel and nerve outgrowth, increases collagen, elastin, and glycosaminoglycan synthesis, as well as supports the function of dermal fibroblasts. GHK’s ability to improve tissue repair has been demonstrated for skin, lung connective tissue, boney tissue, liver, and stomach lining. GHK has also been found to possess powerful cell protective actions, such as multiple anti-cancer activities and anti-inflammatory actions, lung protection and restoration of chronic obstructive pulmonary disease (COPD) fibroblasts, suppression of molecules thought to accelerate the diseases of aging such as NFκB, anti-anxiety, anti-pain and anti-aggression activities, DNA repair, and activation of cell cleansing via the proteasome system. Recent genetic data may explain such diverse protective and healing actions of one molecule, revealing multiple biochemical pathways regulated by GHK.

Keywords: GHK, GHK-Cu, gene profiling, wound healing, COPD, skin regeneration, anti-oxidant, fibrinogen

The human copper-binding peptide GHK-Cu (glycyl-l-histidyl-l-lysine) is a small, naturally occurring tri-peptide present in human plasma that also can be released from tissues in case of an injury. Since its discovery in 1973, GHK-Cu established itself as a powerful protective and regenerative ingredient, which is currently widely used in skin and hair products [1].

Up-to-date, it is established that GHK-Cu is able to:

• -Tighten loose skin and reverse thinning of aged skin
• -Repair protective skin barrier proteins
• -Improve skin firmness, elasticity, and clarity
• -Reduce fine lines, depth of wrinkles, and improve structure of aged skin
• -Smooth rough skin
• -Reduce photodamage, mottled hyperpigmentation, skin spots and lesions
• -Improve overall skin appearance
• -Stimulate wound healing
• -Protect skin cells from UV radiation
• -Reduce inflammation and free radical damage
• -Increase hair growth and thickness, enlarge hair follicle size

Most authors would attribute effects of GHK to its ability to bind copper(II) ions. It was proposed that because of the GHK’s small size and its ability to bind copper, it can play a crucial part in copper metabolism [2]. However, since 2010, a new mechanism has started to emerge. The Broad Institute of MIT and Harvard (Cambridge, MA, USA) has created the Connectivity Map—a publicly available library of transcriptional responses to known perturbagens, substances that modulate gene expression [3]. This tool allowed researchers to investigate genome-wide effects of GHK and establish that GHK-Cu is able to up- and down-regulate a significant number of human genes. Today, it has become possible to connect biological effects of GHK-Cu and its effects on gene expression, to develop a more comprehensive view on GHK’s mechanism of action [4].

The present paper reviews protective and regenerative actions of the GHK-Cu peptide in human skin, as well as new gene data, revealing possible mechanisms behind these actions.

The number of human genes stimulated or suppressed by GHK with a change greater than or equal to 50% is 31.2%. GHK increases gene expression in 59% of the genes, while suppressing it in 41%. For our studies, we used the gene expression results from 50% . This gave the best correlation with our biological data. Table 1 presents an estimate of the number of genes affected by GHK at various cutoff points.

Estimate of number of genes affected by glycyl-l-histidyl-l-lysine (GHK) [5].

Percent ChangeGenes StimulatedGenes Suppressed50–99%1569583100–199%646469200–299%227196300–599%196207600–899%3947900–1199%871200% or more24Open in a new tab

Skin’s ability to withstand damage and repair itself is highest in children and young individuals because of well-functioning repair and protective mechanisms. However, with age, skin’s ability to repair damage declines. GHK content is highest in the plasma of young, healthy individuals. At age 20, the plasma level of GHK is about 200 ng/mL (10−7 M), and by the age of 60, it declines to 80 ng/mL. In the experiment that led to discovery of GHK, plasma from young individuals added to liver tissue obtained from older individuals, caused old liver tissue to produce proteins more characteristic of younger individuals [6].

In the 1980s, Maquart et al. proposed that GHK may be an early signal for skin repair. The GHK amino acid sequence is present in the alpha 2(I) chain of type I collagen, and when damage activates proteolytic enzymes, GHK is released into the site of an injury [7]. A number of experiments established that GHK stimulates synthesis of collagen, selected glycosaminoglycans and small proteoglycan decorin [8,9]. It also modulates activity of key metalloproteinases, which are enzymes that facilitate breakdown of proteins of extracellular matrix, as well as activity of anti-proteases. This suggests a general regulatory effect on protein breakdown in skin, helping to prevent both buildup of damaged proteins and excessive proteolysis [10,11]. Since excessive breakdown of the dermal matrix as well as inadequate removal of damaged proteins can negatively affect skin’s health and appearance, GHK’s ability to regulate both metalloproteinases and their inhibitors can support skin regeneration and improve its appearance.

GHK also demonstrated beneficial effects on skin fibroblasts, which are considered key cells in the skin regeneration process. Fibroblasts not only synthesize structural elements of the dermal matrix but also produce a wide range of growth factors essential for skin repair. GHK, in combination with LED irradiation (light emitting diode irradiation, 625–635 nm), compared with the LED irradiation alone increased: cell viability 12.5-fold, production of the basic fibroblast growth factor (bFGF), 230%, and collagen synthesis, 70% [12].

GHK-Cu has been found to stimulate epidermal basal cells, markedly increasing integrins and p63 expression. The cells’ shape became more cuboidal, which indicates an increase in their stemness [13].

A number of clinical studies confirmed GHK-Cu’s ability to improve appearance of aging skin. A facial cream containing GHK-Cu applied for 12 weeks to the facial skin of 71 women with mild to advanced signs of photoaging increased skin density and thickness, reduced laxity, improved clarity, reduced fine lines and the depth of wrinkles [14].

A GHK-Cu eye cream applied for 12 weeks to around-the-eye area of 41 women with mild to advanced photodamage performed better than placebo and vitamin K cream. It reduced lines and wrinkles, improved overall appearance, and increased skin density and thickness [15].

GHK-Cu applied to thigh skin for 12 weeks improved collagen production in 70% of the women treated, in contrast to 50% treated with the vitamin C cream, and 40% treated with retinoic acid [16]. In addition to improving skin laxity, clarity, firmness and appearance, reducing fine lines, coarse wrinkles and mottled pigmentation, and increasing skin density and thickness, GHK-Cu cream applied twice daily for 12 weeks also strongly stimulated dermal keratinocyte proliferation [17].

With their pilot study for topical application of copper tripeptide complexes in aged skin, Krüger et al. confirmed an increase in skin thickness in the range of the epidermis and dermis, improved skin hydration, a significant smoothing of the skin by stimulating collagen synthesis, increased skin elasticity, a significant improvement in skin contrast and an increased production of collagen I [18,19].

GHK-Cu at 0.01, 1 and 100 nM incubated with human adult dermal fibroblasts increased production of elastin and collagen. GHK also increased gene expression of MMP1 and MMP2 at the 0.01 nM. All concentrations increased TIMP1. The effects of GHK-Cu were also investigated in a randomised, double–blind clinical trial. Female volunteers applied GHK-Cu, encapsulated in nano-lipid carrier twice a day in the course of 8 weeks using either carrier alone or the commercially available peptide Matrixyl® 3000 as controls. Compared to Matrixyl® 3000, GHK-Cu produced a 31.6% reduction of wrinkle volume. Compared to control serum, GHK-Cu reduced wrinkle volume 55.8% and wrinkle depth 32.8% [20].

Multiple animal studies have established the wound healing activity of GHK. It appears that GHK stimulates wound healing through a variety of mechanisms. In rabbit experimental wounds, GHK alone or in combination with high dose helium–neon laser improved wound contraction and formation of granular tissue, as well as increasing activity of antioxidant enzymes and stimulating blood vessel growth [21,22]. Collagen dressing with incorporated GHK (PIC-Peptide Incorporated Collagen) accelerated healing of wounds in healthy and diabetic rats. The treated group displayed higher glutathione (GSH) and ascorbic acid levels, better epithelialization, as well as increased synthesis of collagen and activation of fibroblasts and mast cells in wounds. In healthy rats, treatment of wounds with PIC increased collagen 9-fold [23,24]. GHK-Cu improved healing of ischemic open wounds in rats. Wounds displayed faster healing, decreased concentration of metalloproteinases 2 and 9 as well as of TNF-β (a major inflammatory cytokine) compared with vehicle alone or with untreated wounds [25].

One problem with GHK-Cu is that it is very sensitive to breakdown by carboxypeptidase enzymes. Wounds such as diabetic skin ulcers or bedsores usually develop a “wound serum”, thought to be generated by airborne bacteria settling on the wound. The “serum” rapidly breaks down GHK and probably other growth factors such as TGF (Transforming Growth Factor) and PDGF (Platelet Derived Growth Factor).

Nerve and blood vessel growth is an important factor in skin healing and regeneration.

Sage et al. observed that GHK and related peptides are produced in the course of protein breakdown after an injury from a SPARC protein. SPARC (Secreted Protein Acidic and Rich in Cysteine) is a glycoprotein, mostly expressed in embryonic tissues and in tissues undergoing repair and remodeling. At initial stages of tissue repair, GHK and other peptides containing the GHK sequence (such as KGHK), which are released from SPARC in the course of proteolysis, stimulate new vessels growth. Later in the healing process, GHK and GHK-related peptides inhibit blood vessel growth [26].

When skin healing is inadequate, the healed area is often devoid of sensory abilities. In cell cultures, both Monique Sensenbrenner’s lab (France) and Gertrude Lindler’s lab (Germany) found that GHK stimulates nerve outgrowth, an essential attribute of skin repair. GHK helps restore skin’s innervation through increased production of neurotrophic factors [27,28].

Ahmed and colleagues at the Neurochemistry Lab in Chennai, India wrote that when severed nerves within a rat are placed in a collagen tube impregnated with GHK, there is an increased nerve outgrowth. GHK-Cu increased production of nerve growth factor and the neurotrophins NT-3 and NT-4, sped up the regeneration of nerve fibers from nerve stubs placed in a collagen tube, and increased axon count and proliferation of Schwann cells compared to the control group [29].

When we searched for GHK’s gene activation effects on the Gene Ontology for neurons, we came up with 408 genes up and 230 genes down. So GHK has a significant effect on neurons, but we don’t know exactly what this means. With time, we will be able to analyze the huge amount of data. Table 2 presents the top 10 genes upregulated by GHK and the top 10 downregulated [30].

Genes Relevant to Neurons’ Function upregulated and downregulated by GHK.

Gene Title and Abbreviation (The GENE Database)Percent ChangeOPRM1, 1 opioid receptor, mu 1+1294TP73, 2 tumor protein p73+938KCND1, 3 potassium voltage-gated channel, Shal-related subfamily, member 1+845SLC8A2, 4 solute carrier family 8 (sodium/calcium exchanger), member 2+737CNTNAP2, 5 contactin associated protein-like 2+581STMN3, 6 stathmin-like 3+500LPHN3, 7 latrophilin 3+494ANGPT1, 8 angiopoietin 1+487SYN3, 9 synapsin III+478DPP6,10 dipeptidyl-peptidase 6+448PITX3, 221 paired-like homeodomain 3−541NOTCH3, 222 notch 3−547DLGAP1, 223 discs, large homolog-associated protein 1−547SLIT1, 224 slit homolog 1−553BSN, 225 bassoon (presynaptic cytomatrix protein)−563CELSR1, 226 cadherin, EGF LAG seven-pass G-type receptor 1−647CACNB4, 227 calcium channel, voltage-dependent, beta 4 subunit−672NDN, 228 necdin homolog (mouse)−729EDNRB, 229 endothelin receptor type B−768CHRM2, 230 cholinergic receptor, muscarinic 2−1049Open in a new tab

As animal experiments show, treatment of wounds with GHK leads to elevated levels of antioxidant enzymes. GHK also possesses strong antioxidant and anti-inflammatory actions. GHK inactivated damaging free radical by-products of lipid peroxidation, such as 4-hydroxynoneal, acrolein, malondialdehyde, and glyoxal, protecting cultured skin keratinocytes from ultraviolet (UV)-radiation [31]. GHK was shown to completely block Cu(2+)-dependent oxidation of low density lipoproteins (LDL). Another well-known anti-oxidant, which is also widely used in skin care, superoxide dismutase (SOD1), gave only 20% protection [32]. GHK also prevents damaging effects of lipid peroxidation, by binding its by-products such as acrolein and 4-hydroxynonenal [33,34].

GHK:Cu(2+) reduced iron release from ferritin by 87%. Ferritin in blood plasma can store up to 4500 atoms of iron per protein molecule, which is a well-known catalyst of lipid peroxidation—a chain reaction, which produces a slew of free radicals, leading to DNA, protein and cell membrane damage. Disturbances in iron metabolism contribute to many pathological conditions, including brain damage and neuron death under various neurological conditions. When iron is released from ferritin, it can form an Fe(2+)/Fe(3+) complex and start the chain reaction of lipid oxidation [35].

Successful tissue regeneration requires collaboration of multiple cells, which is orchestrated by various cytokines and growth factors. This means that in order for regeneration to be successful, these molecules have to be produced and released in the right amount and in the right place. This is important because no signal molecule works on its own, but instead engages in a crosstalk, which leads to activation of certain cellular pathways. Among pathways involved in skin regeneration are cellular pathways regulated by TGF-β and integrins [36].  

GHK appears to support remodeling and restructuring of connective tissue, modulating expression of numerous genes, including up-regulation of genes of the TGF-β pathway. This is evident from a study which demonstrated GHK’s ability to reverse expression of key genes, included in a gene signature of COPD—Chronic Obstructive Pulmonary Disease. The expression of 127 genes was altered in COPD patients. More severe emphysema symptoms were correlated with the degree of change in gene expression. Genes whose expression was associated with inflammation were upregulated, and genes involved in tissue remodeling and repair were downregulated significantly. Using the Connectivity Map, a software gene profiling tool developed by the Broad Institute, the researchers sorted through gene expression profiles of biological molecules and came up with GHK as a compound which could reverse changes in gene expression associated with emphysematous destruction, such as decreased activity of genes involved in the TGF-β pathway. GHK was able to change the gene expression pattern to its opposite—activation of the TGF-β pathway.

GHK was then tested in vitro to confirm its positive effects on connective tissue. Lung fibroblasts from COPD patients, which had impaired ability to contract and restructure collagen, were treated with GHK or TGF-β. Both molecules restored function of fibroblasts. They also had an elevated expression of integrin beta 1 [37,38].

The use of GHK-Cu in mice protected their lung tissue from induced acute lung injury (ALI) and suppressed the infiltration of inflammatory cells into the lung. The GHK-Cu also increased superoxide dismutase (SOD) activity while decreasing TNF-1 and IL-6 production through the blocking activation of NFκB’s p65 and p38 MAPK (mitogen activated protein kinase). Mitogen activated protein kinases are kinase enzymes that play crucial part on cellular signaling. P38 MAPK pathways enable cells to respond to a wide range of external stressors, and affect skin differentiation, apoptosis, mobility and gene expression. NFκB p65 activation has been found to be correlated with many diseases of aging and cancer development [39].

Topical steroids, such as cortisone, are often prescribed for treatment of inflammatory cutaneous disorders, can inhibit wound repair, as well as produce skin thinning and other defects [40].

GHK-Cu, administered systemically to mice, rats, and pigs, has protective effects on cortisone—induced inhibition of wound healing [41].

GHK was isolated as a plasma molecule that suppressed fibrinogen. Fibrinogen is best known for its ability to form blood clots. However, it also heavily influences the flow of blood through the microcirculation, where blood acts as a thixotropic fluid, somewhat like toothpaste, flowing under increased pulses of blood pressure, then reverting to a semi-solid gel. Elevated fibrinogen greatly increases the blood viscosity in the microcirculation by increasing red blood cell "stacking" or rouleaux formation. Studies in Germany and Scotland have found that fibrinogen levels are the top risk factor for cardio vascular diseases (CVD). The Prospective Cardiovascular Münster (PROCAM) study followed 5389 men for 10 years. It found that the incidence of coronary events in the top third of the plasma fibrinogen levels was 2.4-fold higher than in the bottom third. Individuals in the top third of levels of low-density lipoprotein (LDL) cholesterol who also had high plasma fibrinogen concentrations had a 6.1-fold increase in coronary risk. Unexpectedly, individuals with low plasma fibrinogen had a low incidence of coronary events even when serum LDL cholesterol was high [42].

The Scottish Heart Health Study followed 10,359 men and women for 2 years, and fibrinogen was the single most powerful risk factor for CVD risk or death and more predictive than lipoprotein cholesterol. The increase in (relative risk) between the highest and lowest fibrinogen levels was 301% for men and 342% for women (CVD death) and 259% for men and 220% for women (death from any cause) [43].

As mentioned above (Park et al.), GHK suppresses the production of Interleukin-6, a main positive regulator of fibrinogen production, both in cell cultures and in mice. As our gene profiling data indicate, GHK downregulates (−475%) the gene for the beta chain of fibrinogen. Since equal amounts of all three polypeptide chains are needed to produce fibrinogen, when synthesis of one of the chain of fibrinogen is suppressed, it will have a general inhibitory effect on fibrinogen synthesis [44].

A major concern for any substance, which activates cell growth and tissue remodeling—is whether it can also trigger cancer. Therefore, it is very important to notice that GHK, which repairs skin, also possesses potent anti-cancer properties.

In 2010, Hong et al. used Broad Institute’s Connectivity Map to find molecules that could inhibit metastatic colon cancer. The Connectivity Map contains expression profiles that were evaluated in five cancer cell lines, in response to 1,309 bioactive small molecules. A search through the database produced only two substances that were able to down-regulate expression of “metastatic” genes— two skin remodeling substances, GHK and the plant alkaloid, securinine. GHK produced the result at a low non-toxic 1 micromolar concentration and securinine at 18 micromolar. GHK suppresses RNA production in 70% of 54 human genes overexpressed in cancer patients, including “node molecules” YWHAB, MAP3K5, LMNA, APP, GNAQ, F3, NFATC2, and TGM2. These molecules play key roles in regulation of important molecular pathways [45]. This shows that GHK is involved in gene regulation of various biochemical pathways, and it seems to be resetting the gene activity back to health, which leads to the improvement of tissue repair [46].

UV-radiation and other damaging factors can damage skin cells’ DNA, which can potentially lead to skin cancer. One of the main protective mechanisms is apoptosis or programmed cell death. Normal healthy skin cells have checkpoint systems to self-destruct if they are synthesizing DNA incorrectly. When apoptosis is inhibited, skin cancer risk greatly increases. Also, apoptosis is the mechanism through which many anti-cancer treatments, including melanoma treatments, work. Some common cosmetic ingredients, such as elderberry extract, can enhance effectivity of cancer treatment by enhancing apoptosis in malignant cells [47].

Matalka et al. demonstrated that GHK, at 1 to 10 nM, inhibited the growth of human SH-SY5Y neuroblastoma cells and human U937 histiocytic lymphoma cells. It also re-activated the apoptosis system, as measured by the caspases 3 and 7. In contrast, GHK stimulated the growth of healthy human NIH-3T3 fibroblasts [48].

In 1983, using a method developed by Linus Pauling’s group [5], we tested the mixture of GHK-copper 2+ plus ascorbic acid (vitamin C) on the growth of sarcoma-180 in mice. This gave a very strong suppression of the cancer. These results remained unpublished until 2014, when we could supplement these findings with gene data. We used the Broad Institute Connectivity Map to investigate the effect of GHK on genes relevant to cancer growth. GHK upregulated the expression of 10 caspase and caspase-associated genes. It also affected 84 genes associated with DNA repair and other processes, relevant to anti-cancer effects. The anti-cancer activity of GHK may be linked to its known tissue remodeling effects [49].

The ubiquitin proteasome system (UPS) is a system that processes and clears damaged proteins. When this system is not functioning properly, damaged proteins may start accumulating. Aging is associated with decreased activity of the ubiquitin proteasome system. So far, there are no effective therapies to increase the UPS activity. Recent work has demonstrated that proteasome activation by either genetic means or use of compounds slows down aging [50].

We performed a search, using gene titles containing “ubiquitin” or “proteasome”. GHK strongly stimulates the gene expression of the UPS system, increasing activity of 41 genes and suppressing 1 gene. See Table 3.

GHK’s Effect on Gene Expression relevant to the Ubiquitin/Proteasome System [4].

Gene Title-Gene-Expression IncreasedPercent Change1ubiquitin specific peptidase 29, USP29+10562ubiquitin protein ligase E3 component n-recognin 2, UBR2+4553gamma-aminobutyric acid (GABA) B receptor, GABBR1+3104ubiquitin specific peptidase 34, USP34+1955parkinson protein 2, E3 ubiquitin protein ligase (parkin), PARK2+1696ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), UBE2I+1507ubiquitin protein ligase E3 component n-recognin 4, UBR4+1468ubiquitin protein ligase E3B, UBE3B+1169ubiquitin specific peptidase 2, USP2+10410ubiquitin-like modifier activating enzyme 6, UBA6+10411ubiquitination factor E4B (UFD2 homolog, yeast), UBE4B+9912ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), UBE2M+9213Ubiquitin-like modifier activating enzyme 7, UBA7+8814HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1, HECW1+8115proteasome (prosome, macropain) 26S subunit, ATPase, 3, PSMC3+8116ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast), UBE2D1+7917proteasome (prosome, macropain) subunit, beta type, 2, PSMB2+7918ubiquitin protein ligase E3 component n-recognin 5, UBR5+7719ubiquitin specific peptidase 21, USP21+7620OTU domain, ubiquitin aldehyde binding 2, OTUB2+7621proteasome (prosome, macropain) inhibitor subunit 1 (PI31), PSMF1+7522ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast), UBE2H+7323ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast), UBE2N+7224ubiquitin carboxyl-terminal hydrolase L5, UCHL5+7125ubiquitin specific peptidase 6 (Tre-2 oncogene) pseudogene, LOC220594+7126proteasome (prosome, macropain) 26S subunit, non-ATPase, 13, PSMD13+7027ubiquitin associated protein 1, UBAP1+7028ubiquitin-conjugating enzyme E2B (RAD6 homolog), UBE2B+6929TMEM189-UBE2V1 readthrough, TMEM189-UBE2V1+6730proteasome (prosome, macropain) 26S subunit, non-ATPase, 1, PSMD1+6431proteasome (prosome, macropain) 26S subunit, non-ATPase, 3, PSMD3+6432ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila), ARIH1+6133BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase), BAP1+6034ubiquitin interaction motif containing 1, UIMC1+6035ubiquitin associated protein 2-like, UBAP2L+5736ubiquitin protein ligase E3 component n-recognin 7 (putative), UBR7+5637ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast), UBE2G1+5438itchy E3 ubiquitin protein ligase homolog (mouse), ITCH+5439ubiquitin-conjugating enzyme E2D 4 (putative), UBE2D4+5140proteasome (prosome, macropain) 26S subunit, non-ATPase, 10, PSMD10+5041WW domain containing E3 ubiquitin protein ligase 1, WWP1+5042ubiquitin-like 3, UBL3+50Gene Title-Gene Expression SuppressedPercent Change1ubiquitin associated and SH3 domain containing A, UBASH3A−89Open in a new tab 

According to Broad Institute data, UPS genes changed at least 50% UP or DOWN. GHK increased gene expression in 41 UPS genes while suppressing 1 UPS gene. This should have a positive effect on this system.

GHK has potent anti-pain, anti-anxiety (anxiolytic) and anti-aggression actions.

Anti-pain effects were measured by determining how long it took for mice to lick their paws after being placed on a mildly-hot plate. Here, GHK reduced pain at a dose of 0.5 milligrams/kilogram. GHK has a physical structure similar to cimetidine which is often used to reduce pain in humans [51,52].

The anti-anxiety, anti-pain and anti-aggression actions were found in rats. When rats are afraid, they try to hide. But within 12 min of intraperitoneal injection of GHK at 0.5 micrograms/kilogram into rats in a testing cage built as a maze, the amount of time the rats spent exploring more open and lighted areas of the maze increased, and the time spent immobile (the freeze reaction) decreased, which indicated a reduction of fear and anxiety [53]. The same occurred in a test “open field”, where the rats spent less time hiding and more time exploring the area [54].

Likewise, the anti-aggression actions were found in rats. For the experiment, two rats are placed in a small cage and then given small electrical shocks, which produces anger in the rats and led to physical attacks on the other rat. The attacks were reduced 5-fold, when rats were placed into an agression-stimulating environment 12 min after the injection of 0.5 micrograms GHK per kilogram of body weight. If scaled up for human weight, this suggests that a similar effect might be induced in humans by 35 micrograms of GHK, which is a very low and safe dosage [55].

Studies of GHK passage through the skin by Howard Maibach’s laboratory suggest that it may be possible to easily pass an adequate amount of GHK-Cu through the skin to reduce anxiety, and possibly pain [56].

A manual search of genes affected by GHK found that seven anti-pain genes increased and two genes decreased. The results are presented in the Table 4.

GHK and Genes Associated with Pain [30].

Gene Abbreviation, Name (GENE Database)Percent ChangeComment (GENE Database)ORPM1, opioid receptor mu 1+1294the principal target of endogenous opioid peptides such as beta-endorphin and enkephalins.CCKAR, cholecystokinin A receptor+190regulates satiety and the release of beta-endorphin and dopamine.CNR1, cannabinoid receptor 1+172involved in the cannabinoid-induced CNS effectsSIGMAR1, sigma non-opioid intracellular receptor 1+155a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines.PNOC, prepronociceptin+150A receptor for proteins involved in regulation of pain sensitivityOXT, oxytocin/neurophysin I prepropeptide+136A precursor protein for oxytocinGRIA3, glutamate ionotropic receptor AMPA type subunit 3−126Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brainOPRK1, opioid receptor kappa 1−119a receptor for various synthetic opioidsOpen in a new tab

Psychological stress is an adaptive response that can be deleterious under certain conditions. Stress and anxiety delay epidermal barrier recovery, impair skin immune function, increase inflammation and oxidative stress. It causes activation of the hypothalamus-pituitary-adrenal (HPA) axis and the sympathetic nervous system. Skin is affected by molecules that are released during stress, such as neuropeptides, hormones, and cytokines [57]. This makes analgesic and anxiolytic effects of GHK an important part of its skin-related effects. Studies show that reducing anxiety and psychological stress can have a positive effect on chronic skin conditions such as psoriasis and atopic dermatitis [58]. Chronic stress can also impede wound healing, affecting recovery from plastic surgery and other invasive cosmetic procedures [59,60]. We believe that the GHK molecule evolved as the first response to an injury, and as such it is not surprising that it possesses analgesic and anxiolytic effects. Lowering the level of stress-related hormones and cytokines could help animals reduce inflammation and increase the chance of surviving the injury.

GHK-Cu can penetrate the stratum corneum, which ensures its activity in cosmetic formulations [61,62]. However, to increase GHK’s penetration, it is advisable to use liposomes, including nanosized liposomes [63].

GHK is safe and very inexpensive. It can be easily incorporated into skin protective formulations, such as sunscreens and daytime creams and serums, as well as anti-wrinkle formulations. Because of its ability to improve wound healing, it is recommended after plastic surgery, chemical peels, dermabrasion, laser treatment, and so on. It will be very useful in a clinical setting and in assisted living senior facilities as a wound dressing, especially for diabetic and ischemic wounds.

Its safety record and its ability to reverse gene expression back to health warrant its use as a dietary supplement to support health and vitality of skin, hair and the entire body.

GHK is a small molecule, which possesses a surprisingly wide range of health-promoting qualities, while new studies are still revealing an even broader scope of GHK’s biological effects.

In the past, the wound healing, tissue remodeling, angiogenesis-promoting, cell-growth stimulating, anti-inflammatory and anti-oxidant actions of GHK were attributed to its unique relationship with copper. Copper is a transitional metal that is vital for all eukaryotic organisms from microbes to humans. Since it can be converted from oxidized Cu(II) to reduced Cu(I) form, it functions as an essential co-factor in a multitude of biochemical reactions involving electron transfer. A dozen enzymes (cuproenzymes) use changes in copper oxidation states to catalyze important biochemical reactions, including cellular respiration (cytochrome c oxidase), antioxidant defense (ceruloplasmin, superoxide dismutase (SOD), detoxification (metallothioneins), blood clotting (blood clotting factors V and VIII), and the connective tissue formation (lysyl peroxidase). Copper is required for iron metabolism, oxygenation, neurotransmission, embryonic development and many other essential biological processes [64].

Even though the copper hypothesis of GHK’s mode of action is still valid, we feel that it doesn’t explain the gene modulating effects of GHK-Cu. Therefore, in light of the new gene data, a new model of GHK-Cu action is needed, which will require collaboration of researchers from different fields.

As new gene profiling studies reveal, GHK with and without copper affects a large number of genes related to an organism’s response to stress and injury (tissue remodeling, anti-oxidant, anti-inflammatory, anti-pain, anti-anxiety, blood vessel growth, nerve outgrowth, anti-cancer action). GHK sequence is included in the collagen molecule, and SPARC protein and GHK is naturally released after an injury due to protein breakdown.

It is now known that some age-related changes in gene expression are not permanent and can be reversed. Studies show that regular physical exercise of older humans, as little as 30 min daily three times a week, can reset mitochondrial human DNA to a gene expression more like that of a younger person. Other procedures such as healthy diets, wine consumption, and flavonoid supplements are able to modify activity of certain genes, and various types of mediation and anti-stress methods are recommended to improve gene expression [65,66]. However, most biological compounds tested for their effects on gene expression using computer-based tools often lack supporting biological data. GHK has been extensively studied for over four decades and its safety and biological effects has been confirmed in cell, tissue and animal studies. In our opinion, the COPD study (Campbell et al.) is the most representative in this aspect, because not only were the gene signatures derived from areas with histologically confirmed pathology, but also GHK’s effects on affected lung tissue were tested in vitro and their correlation to gene effects was well-established [37].

GHK is a safe, inexpensive, extensively studied compound that has a wealth of positive and health-promoting effects in many tissues and systems. It has been widely used in anti-aging and cosmetic products in humans for decades without any adverse effects, and can be easily incorporated in creams, liposomes, dermal patches or delivered through microneedles. At present, it is not formulated into dietary supplements, so in our opinion, developing and testing GHK-based products for internal use to support health of elderly populations and as a complimentary therapy in cancer treatment is one possible direction for future research. Based on both biological and gene data, GHK also has the potential to be developed into an anti-anxiety and anti-pain supplemental treatment, and it may be an essential component in a future complex approach to COPD therapy.

Authors want to thank Germaine Pugh and Cassia McClain for their invaluable help in preparing the manuscript.

This research received no external funding.

The authors declare no conflict of interest.

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ISSN : 2456-6373   

DOI: https://doi.org/10.22259/ijrsmhs.0605002 

Phototherapy Induced Metabolism Change Produced by the 

LifeWave X39 Non-transdermal Patch 

Melinda H. Connor, D.D., Ph.D., AMP, FAM*1, Caitlin A. Connor, MAcOM, DAOM2, 

Naran Gombusuren, Ph.D.3, Jens Eickhoff, Ph.D.4, Nathan Anderson, MACOM, LAc.5, Marsha Perry, RN, MA6, Leonard Peugh, MAcOM7 

1Founder, Earth Songs Holistic Consulting 

2Post Doctoral Diploma Candidate, Health Sciences Research, Rewley House, Oxford University, UK 

3Global Operations Manager, Serametrix/Caprion 

4Senior Scientist, Biostatistics & Medical Informatics, University of Wisconsin Madison 

5Dean of Clinical Education, Arizona School of Acupuncture and Oriental Medicine 

6Study Manager, Earth Songs Holistic Consulting 

7Senior Research Assistant, Earth Songs Holistic Consulting 

*Corresponding Author: Melinda H. Connor, D.D., Ph.D., AMP, FAM, Earth Songs Holistic 

Consulting, 31907 South Davis Ranch Rd. Marana, AZ 85658, USA 

Purpose: To determine X-39 patch impact in stimulation of Copper peptide biosynthesis and bio-available amino acid levels, neurotransmitters production, memory, sleep quality, vitality, muscle relaxation and blood pressure. 

Materials: Biography Infinity physiology suite: Heart rate variability (HRV), GSR, EMG, EKG, blood volume pulse (BVP), temperature and respiration. Questionnaires: Marlow-Crowne, Global Mood Scale, Pittsburg Sleep Quality Index, Arizona Integrative Outcomes scale. WAIS III memory test. Amino acid and neurotransmitters testing of urine. 

Method: Subjects were recruited (age 40 - 81), consented, randomized and scheduled. Data taken day 1, day 2, and day 7 except Marlow-Crowne taken day 1 and day 7. 

Results: Improvements in short term memory p<0.001, sleep quality p<0.04, vitality p<0.03 day 2 and p<0.08 at day 7. Blood pressure change in VLF on day 7 at p<0.02, respiration on day 7 at p<0.04. Increase in amino acids: Creatinine, Normetanephrine, methionine, homocystine, isoleucine, glutamine, cysteine, 5-hydroxytrytophane, β-aminobutyric-acid. 

Conclusion: The results of the double blind randomized controlled trial of 50 subjects with mean age 63 years, using the LifeWave non-transdermal X-39 phototherapy patch worn 8-12 hours per day for seven days produced an increase in 8 amino acids at significant levels. The study showed, there was an improvement in short term memory as measured by the WAIS III memory test at significant levels over 7 days, in Quality of Sleep at significant levels within 24 hours and a self reported increase in vitality at near significant levels in 7 days as measured by the Arizona Integrative Outcomes scale. 

Our findings suggest this patch is not only stimulating the biosynthesis of copper-peptide production, but also increases neurotransmitters production and improves metabolism. Additional studies could address underlying mechanisms of action to the phototherapy process and longer periods of study might be explored for additional potential physical changes and longevity of the demonstrated changes. 

Keywords: Photobiology, Photobiomodulation, Amino Acid, Metabolism 

The Lifewave X39 non-transdermal patch focuses on stimulating the copper tripeptide 

International Journal of Research Studies in Medical and   Health Sciences V 5 ● 2021 

GHK- Cu [1,2,3]. “Copper tripeptide-1(GHKCu) is a small protein composed of the three amino acids glycine, histidine, and lysine combined in a specific geometric configuration with the physiologically beneficial mineral (copper)” [4]. The goal of this research was to determine X-39 patch impact in stimulation of Copper peptide biosynthesis and bio-available amino acid levels, neurotransmitters production, memory, sleep quality, vitality, muscle relaxation and blood pressure. This tripeptide was first isolated from human plasma albumin in 1973 by Dr. Loren Pickart. Pickart, noticed differences in the levels of fibrinogen based on age. He additionally noticed that these differences stopped when the older liver cells were incubated in blood from younger individuals. “In 1977, David Schlesinger of the Harvard University Chemistry Department confirmed that the growth modulating peptide isolated by Pickart was a glycyl-L-histidyl-Llysine peptide”[5]. It is interesting to note that this peptide has also been found in saliva, urine, and collagen. Additional research has established the strong affinity the GHK peptide has for copper, and exists in two forms, as this was not covered in the initial experiment. These two forms are GHK and GHK-Cu. It is also important to mention that none of the research around GHK has ever found it to cause an issue [4]. 

Research has identified that the peptide is used to signal the beginning of the natural repair process. This benefit has specifically been documented through research for post-laser or surgical wounds, ischemic, burns, skin or hair transplants, and diabetic ulcers. “Diabetic wounds healed three times faster in the presence 

of Copper tripeptide-1. Time to reepithelialization is shortened” [4]. The tripeptide has also been demonstrated to improve tissue remodeling. “It increases keratinocyte proliferation and normal collagen synthesis, improves skin thickness, skin elasticity and firmness, improves wrinkles, photodamage and uneven pigmentation, improves skin clarity, and tightens protective barrier proteins” [4]. This has an impact on both scars and other effects of damage to the skin, and natural aging processes. The effects of tissue remodeling also appear to have an impact on cancerous cells. “The fact that GHK was able to suppress 70% of genes involved in the development of an aggressive metastatic form of colon cancer indicates that GHK is capable of the regulation of various biochemical pathways on a gene level and it seems to be resetting the gene activity back to health, which leads to the improvement of tissue repair” [6]. 

GHK-Cu also has a demonstrated impact on other organs in the body after they have been damaged. “A collaborative study conducted by scientists from Boston University, University of Groningen, University of British Columbia, and University of Pennsylvania established that the GHK peptide reverses the gene expression signature of COPD, which is manifested by emphysema, inflammation, lung tissue destruction, and significant reduction of lung capacity” [6]. It is also important to note that 

“…the level of GHK is about 200 ng/mL(10−7M) at age 20 and it declines to 80 ng/mL by age 60” [6]. This likely explains the increasing effects of aging. It would also suggest that increased levels over time of GHKCu would have a positive effect on both life expectancy and aging. 

While phototherapy has been defined as “the use of ultraviolet (UV) light for its healing effects” 

[7], the LifeWave patches have been specifically developed to reflect light in the infrared and visible light bands back onto the skin where the patch has been placed. Supported by normal electro-dermal skin conductance [8,9], the human body gives off a number of materials biochemically including particulate release, gas emission, ultraviolet, infrared, near infrared, and visible spectrum light. This then stimulates the area of skin, producing improved physiological effects. Variations on phototherapy have been used for at least 100 years. In that time there has been little evidence of negative side effects. This suggests that this is a relatively untapped option for healing with relatively few risks. 

International Journal   of Research Studies in Medical and Health Sciences V6 ● I5 ● 2021 

This study was done as a whole systems research randomized controlled double blind trial of 50 participants. Human studies ethics approval for this research was given NFFEH 0331-19-02. Participants were recruited using flyers, emails and radio announcements. A sample of convenience drawn from those who responded and met inclusion/exclusion criterion of men and women age 40-81 with no major mental health issues who were then randomized into control and     

active groups via computer and to patch placements (GV14, CV6). Patches used in the trial were mailed prepackaged as groups A and B and the blind was not broken until after the statistical analysis was done. Participants were scheduled for the same time each day for each of the data points (day1/baseline, day2/24 hours, day 7). Participants were consented, and data was taken in the following order: questionnaires, urine sample, physiology measures. On day one the participant was given 9 days supply of the patches for use in the study. A one month supply of the active patch was given at the end of the week's participation and $20 toward the participants time and gasoline. 

The trial was conducted at the Arizona School of Acupuncture and Oriental Medicine clinic in Tucson, AZ. 

All X39 patches are sealed so that none of the substances in the patch actually penetrate the skin. This allows for consistent patch promotion of the light flow during the period of patch application. There is sufficient evidence of electrical-dermal response in orthopedic research to use previously measured acupuncture points as "strategic conductors of electromagnetic signals" [10]. Two points were selected for this research so the process of patch placement could be standardized across the participant population. Earlier studies of this patch selected acupuncture/acupressure points designated GV14 and CV6 [2, 3] and based on pilot results, the same patch placement was selected for consistency. Participants were randomized by computer into groups and the point usage was selected based on the randomization. 

Sabre Sciences Inc. conducted the urine analysis using their HPA1 metabolic panel using LCMS/MS tandem mass spectrometry. The metabolic panel measures excreditory level of amino acids and their metabolites, including catecholaminergic, serotonergic, glutamatergic pathways and metabolites, and trans-sulfuration and histidine pathways. Three urine data points were taken on each subject before 10am each of baseline/day1, at 24 hours/day 2 and on day 7. Samples were frozen and then shipped to the Sabre Science laboratory in Carlsbad, CA each day for analysis. 

The Thought Technology Biography Infinity physiology suite was used for six minute measures of heart rate variability (HRV), galvanic skin response (GSR), EMG, EKG, blood volume pulse (BVP), temperature and respiration at each data point. 

Questionnaires and memory test were administered at each data point with the exception of the Marlow-Crowne which was administered day one and day seven. 

The Marlowe Crowne is a 13 Item true/false short likert scale that measures political correctness. This instrument was selected to confirm accuracy of the data. The instrument takes about 2 minutes to do for most 

participants. (Norming: [11].) Pittsburg Sleep Quality Index 

Normed by Cole et al (2006) this scale is a mix of quantitative questions and five likert scale questions 0-3 addressing the participants quality of sleep. 

Arizona Integrative Outcome Scale, Visual Analogue Scale (AIOS-VAS) for Vitality The AIOS- VAS (Normed: [12]) rates subject’s “overall sense of well-being and vitality.” It uses a 100mm one-line visual analogue scale on which the participant notes their sense of well being with an x on the line. 

A subsection of the WAS III was administered which looks at the level of short, mid and longer term memory. The test was administered concurrent to the filling out of other questionnaires. Ten numbers which were taken from a randomized list were repeated without inflection three times to each participant. Participants were asked to immediately repeat the numbers back at the end of the third repetition, then again asked to repeat the numbers back at 10 minutes and at twenty minutes. Count was taken of the number of correct digits in the order originally given. Count was stopped when an error in the order was made. Global Mood Scale 

Denollet normed the Global Mood Scale in 

1993. It includes a twenty item, five factor likert scale that assesses participants current mood and a one item, ten factor likert scale assessing well being at the current moment. It was selected both as a redundant measure comparison to the AIOS-VAS and an overall assessment of mood. 

All data results were entered into spread sheets and they were then analyzed for significant results. Questionnaires were analyzed for mean and standard deviation and stratified by assessment time point. Paired t-test or nonparametric Wilcoxon Signed Rank tests were then done. Physiology measures were summarized stratified across the 6 study epochs looking at means and standard deviation. Pre - post changes were evaluated using a paired ttest. Distribution assumptions were then verified using normal probability plots. The values for p are two-sided and statistical significance was defined as p<0.05. The metabolic analysis included stratification by assessment point and summary means and standard deviation. Analysis was done including changes from baseline to day 2, day 2 to day 7 and day 1 to day 7. These were evaluated using Paired t-test or nonparametric Wilcoxon Signed Rank tests. 

This was a double blind randomized controlled trial with a sample of convenience and which had a seven day intervention period. While this was a sample of convenience participants were recruited through five different methods including: radio announcements, email, posting in cafe's and at university center's, prior study participant announcement lists and through area community groups. So while this could be considered a study weakness, the range of recruiting methods insured a diversity of population who decided to participate. There were 26 individuals in the Active group and 24 in the Control group, with an overall mean age of 63 and a range of 40-81 years. The Active groups mean age matched the overall mean, while the Control group was slightly lower at 62 years. Despite the limitations of smaller participant sample size (N=50) in this study, there were several interesting significant changes, as well as some changes of near significance within the study test results. There were two adverse events, where two individuals in the study had headaches while using the patches. These headaches took place during monsoon season, and both were individuals who had a previous history of barometric pressure triggered migraine headaches. The IRB reviewed the cases and determined that they were both consistent with the individuals past history and had nothing to do with the product. Two individuals were also dropped from the study when they did not complete the study visits. 

The specific impact of research location should also be recognized. Aside from the barometric pressure effects of monsoon season, this study largely took place over the summer in Arizona. This meant that participant hydration was a constant issue, especially with the necessity of a urine sample. As such, it was determined that water had to be available and was offered to each participant at the beginning of each study appointment. Included are the significant and some near significance findings for the LifeWave non-transdermal X39 phototherapy patch. 

The urine analysis data showed an increase in amino acids: Creatinine, Normetanephrine, methionine, homocystine, isoleucine, glutamine, cysteine, 5-hydroxytrytophane, β-aminobutyricacid. These are important findings as branched chain amino acids like isoleucine, which is constantly oxidized in muscle, methionine and homocystine, are major ingredients of transsulfuration pathway. This pathway is responsible for methyl donor S-adenosylmethionine (SAMe) production. SAMe is readily donates methyl groups to other substances, enabling cardiovascular, neurological, reproductive and detoxifying systems. 5-hydroxytrytophane is major building block of important neurological monoamine neurotransmitter, serotonin, which later methylates into melatonin, and is a main hormone which regulates sleep-wake cycle. Normetanephrine is a norephinephrine break down product in dopamine pathway and is major hormone of neuromodulatory system. 

Table 1: Comparisons of amino acid concentrations between Control group vs. Active group at day baseline (1), day 2 (2) and day 7 (7) All amino acids concentration results were summarized in terms of means and standard deviations (SD), stratified by group (Control vs. Active) and assessment time point (1, 2, 7). Absolute changes from baseline (1) to 2 and baseline (1) to 7 were evaluated using a paired t-test or nonparametric Wilcoxon signed rank test. All reported p-values are two-sided. 

All questionnaire scores were summarized in terms of means and standard deviations (SD), stratified by group (Control vs. Active) and assessment time point (1, 2, 7). Absolute changes from baseline (1) to day 2 and baseline (1) to day 7 were evaluated using a paired t-test or nonparametric Wilcoxon signed rank test. All reported p-values are two-sided. 

Questionnaire outcomes: 

• WAIS III (three subscales #Short, # Mid, #Long) 

• Global Mood Scale (two subscale: Positive Affect (PA), Negative Affect (NA)) 

• Pittsburgh Sleep Quality Index (total score – higher score indicates worse sleep quality) 

• Marlow-Crowne (total score) 

• AIOS-VAS (total score) 

Within the questionnaire results the shifts in sleep were particularly interesting, as they were significant in both groups, in opposite directions. The Active group had a significant improvement in sleep, and the control group had a significant decline in sleep. Vitality also showed a particularly interesting shift, with significant improvement within 24 hours which dropped slightly at day 7. The Global mood scale showed a change in negative affect in both groups. Additionally the Marlow-Crowne showed no significance so that responses on the questionnaires were not as a result of the desire to provide socially correct or desired answers. 

Table 4: Comparisons of significant absolute changes in GMS subscale scores (PA-Positive Affect, NA-

Negative Affect) from baseline (1) to day 2 and baseline (1) to day 7 between Control group vs. Active group 

Physiological measures showed significant normalization in blood pressure in VLF p<0.02 and near significance in LF p<0.09 and power p<0.06. Average respiration became deeper by day 7 at p<0.04 and neck and shoulder muscles showed improved relaxation effects by day 7 at p<0.08. 

Table8: Summary of significant and near significance physiological parameters, stratified by time (day) and group. Since the physiology parameters were non-normally distributed, all parameters were summarized in terms of medians and interquartile ranges (IQR). Changes from run 1 (baseline) to run 3 were evaluated using a nonparametric Wilcoxon signed rank test. Comparisons between groups were conducted using a nonparametric Wilcoxon rank sum test. All reported p-values are two-sided and P<0.05 was used to define statistical significance.  

1: IQR: Interquartile range (25th to 75thpercentile) 

2: P-value for comparison between Control group vs. Active group 

Average†: Average across Epoch 1-6 

A comparison of this studies results with the pilot study showed some interesting differences. The first was the change in the level of significance in memory improvement. While the pilot study showed a near significant improvement in short-term memory, the double blind showed very clear significance, p<0.001, as well as near significance in mid-term memory. It would be very interesting to see if this improvement continued to strengthen with a longer intervention period or a population with a mean age of 70+. 

Of note, was the difference in which amino acids were significant between the pilot and the current study. The amino acids which has significance were further down the catecholaminergic, serotonergic, and glutamatergic pathways that the significant findings of the pilot study. This may be due to the difference in median age, with the double blind having an older median population or may be due to utilization by the body when higher concentrations of the amino acids were available for use. It should also be noted that while we saw more prevalent amino acid changes tied to antioxidant events in the pilot study, the data from the double blind shows an overall rebalancing of the gut and overall improvement in gut performance. 

It would be interesting to determine how long and at what continuing rate changes to gut performance, physiological and overall wellness support would be produced by wearing the LifeWave X-39 patch. Longer periods of study such as six to twelve weeks including comprehensive blood and metabolism testing might be considered in the future. 

International Journal   of Research Studies in Medical and Health Sciences V6 ● I5 ● 2021

The results of the double blind randomized controlled trial of 50 subjects on the LifeWave non-transdermal X-39 phototherapy patch worn for 8-12 hours per day for seven days produced an increase in 8 amino acids at 10 significant levels over the 3 time periods. There was additional increase in 3 amino acids at near significance. These specific changes served to rebalance the gut toward a positive homeostatic balance. There was an increase in short term memory as measured by the WAIS III memory test at significant levels over 7 days. There was an improvement in Quality of Sleep at significant levels within 24 hours and a self reported increase in vitality at near significant levels in 7 days as measured by the Arizona Integrative Outcomes scale. Given the mean age of the population at 63 years this is a substantial improvement in overall quality of life. Additional studies could address underlying mechanisms of action to the phototherapy process and longer periods of study might be explored for additional potential physical changes. 

Funding for the study was provided by: LifeWave Corp. 

All author salaries for the research were provided by LifeWave. No author has a commercial interest in the company of any kind. 

[1] Connor, C., Connor, M., Yue, D., Eickhoff, J., Wagner, S., et al. (2021) Double-Blind Testing of the Lifewave X39 Patch to Determine GHKCu Production Levels. Internal Med Res Open J Volume 6(1):1-3. 

[2] Connor, C., Connor, M ., Yue, D., Chang, CL., Eickhoff, J., Wagner, S. "Changes in Tripeptides Produced by the LifeWave X-39 patch", International Journal of Healing and Caring Online, May 3, 2020 

[3] Connor, M., Connor, C., Gombosuren, N., Eickhoff, J., "LifeWave X39 Pilot Demonstrates Light Triggered Changes", International Journal of Healing and Caring Online, May 2020 

[4] DeHaven, C., (2014) Copper Tripeptide-1. Science of Skincare. 

[5] Schlesinger, DH; Pickart, L; Thaler, MM (1977). "Growth-modulating serum tripeptide is glycyl-histidyl-lysine". Cellular and Molecular Life Sci 33 (3): 324–325. doi:10.1007/BF0200 2806. 

[6] Pickart, L., Vasquez-Soltero, J., Margolina, A. (2015) GHK Peptide as a Natural Modulator of 

Multiple  Cellular  Pathways  in  Skin 

Regeneration. Hindawi Publishing Corporation 

BioMed Research International Volume 2015, Article ID 648108, 7 pages http://dx.doi.org/10.1155/2015/648108. 

[7] Kakimoto, C., (2017) What is phototherapy, and how does it work? https://www. dermatologistoncall. com/blog/what-isphototherapy-and-how-does-it-work/ 

[8] Flick, A. B., “Silver and Wound Healing”, CAM Research Symposium, Hershey Medical School, Hershey PA, July 2004. 

[9] Becker, R., Selden, G., “The Body Electric”, William Morrow Pub., New York, NY, 1985. 

[10] Feinstein, D., “Rapid Treatment of PTSD: Why Psychological Exposure with Acupoint Tapping May Be Effective”, Psychotherapy: Theory, Research, Practice, Training. 47(3), 385-402, 2010, American Psychological Association. 

[11] Reynolds, W. (1982) Development of reliable and valid short forms of the Marlowe-Crowne Social Desirability Scale. Journal of Clinical Psychology. January 1982. 

[12] Bell, I., Cunningham, V., Caspi, O., Meek, P., Ferro, L., (2004) “Development and validation of a new global well-being outcomes rating scale for integrative medicine research.” BMC Complementary and Alternative Medicine. 

2004; 4: 1. 

Citation: Melinda H. Connor et al, “Phototherapy Induced Metabolism Change Produced by the LifeWave X39 Non-transdermal Patch”, International Journal of Research Studies in Medical and Health Sciences. 2021; 6(5):8-14. DOI: https://doi.org/10.22259/ijrsmhs.0605002 

Copyright: © 2021 Melinda H. Connor et al, This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 

[1]:p-pvalue for evaluating changes from baseline to 2 and baseline to 7 within each arm 

[2]:p-value for comparing changes from baseline to 2 and 7 between arms 

Table 6:Comparisons of Marlow-Crowne scores between Control group vs. Active group at time baseline (1) and day 7 (7) 

[1] Reynolds, W. (1982) Development of reliable and valid short forms of the Marlowe-Crowne Social Desirability Scale. Journal of Clinical Psychology. January 1982. 

[2] Bell, I., Cunningham, V., Caspi, O., Meek, P., Ferro, L., (2004) “Development and validation of a new global well-being outcomes rating scale for integrative medicine research.” BMC Complementary and Alternative Medicine. 

2004; 4: 1. 

Citation: Melinda H. Connor et al, “Phototherapy Induced Metabolism Change Produced by the LifeWave X39 Non-transdermal Patch”, International Journal of Research Studies in Medical and Health Sciences. 2021; 6(5):8-14. DOI: https://doi.org/10.22259/ijrsmhs.0605002 

Copyright: © 2021 Melinda H. Connor et al, This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 

Your body is an amazingly complex concert of miracles that work synergistically together to bring homeostasis and balance. 

Acupressure is a traditional long-standing practice of healing that stimulates the flow of energy to promote well being. It is based on the same principles as acupuncture, but instead of using needles, acupressure relies on manual pressure on specific body points. X39 patches use pressure by stimulating the skin with infrared light from your body.

Acupressure is part of the Traditional Chinese Medicine (TCM) modality of health and involves working with the body’s meridian system. This system is a network of energy channels through which vital life force, or Qi, flows. By applying stimulation with LifeWave patches to specific acupoints along meridians, energy flow blockages can be released, promoting balance and healing.

The X39 patch uses patented organic nanocrystal technology and works through photobiomodulation (a type of phototherapy) and acupressure. When applied, it reflects specific wavelengths of infrared light from your body at specific acupoints, which then signals your body to work toward optimal energy flow. 

A good example of how phototherapy works is with the sun. When sunlight hits your skin, your body is signaled to create vitamin D. With X39, your body is reflecting back its own infrared light making it a very simple and easy way to gain optimal health.

When the body produces copper peptides (GHK-Cu), mitochondria are involved in regulating the balance between the amount and quality of GHK-Cu that is produced. Mitochondria provide the necessary energy and metabolic support for GHK-Cu proliferation (growth and increase).

GHK-Cu stimulates blood vessel and nerve outgrowth (regeneration), increases collagen, elastin, as well as supports the function of dermal fibroblasts, which are the main cell type present in skin connective tissue (dermis).

X39 has been clinically proven to significantly increase GHK-Cu (copper peptides) in the body in only one week of use.

Research Open Internal Medicine Research - Open Journal

Research Article 

Double-Blind Testing of the Lifewave X39 Patch to Determine GHK-Cu Production Levels

Caitlin A Connor1, Melinda H Connor2*, David Yue3, Jens Eickhoff4, Susan Wagner5and Amy Chang6

1Post-Doctoral Fellow, Rewley House, Oxford University, UK

2Founder, Earth Songs Holistic Research and Consulting, USA

3CEO, HT-Labs a division of Axis Pharm, San Diego CA, USA

4Senior Scientist, Biostatistics & Medical Informatics, University of Wisconsin Madison, USA

5Professor, Arizona School of Acupuncture and Oriental Medicine, USA

6Senior Research Associate, HT-Labs a division of Axis Pharm, San Diego CA, USA

*Corresponding author: Melinda H Connor, Earth Songs Holistic Research and Consulting, 31907 South Davis Ranch Rd, Marana, AZ 85658, USA; Tel: (520)609-

1765; E-mail:melinda_connor@mindspring.com

Received: February 23, 2021; Accepted: March 02, 2021; Published: March 07, 2021

Purpose: To determine if the LifeWave X39 non-transdermal photobiomodulation active patch would show improved production of GHK-Cu over controls in a double blind randomized controlled trial.

Materials: BD Vacutainer Safety Loc Blood Collection sets with Pre-attached holder sized 21GX0.75 or 23GX0.75 and lavender top tubes. Kendro Sorvall Biofuge Centrifuge 75005184+ and AB Sciex API4000 Qtrap. Analysis software included: Qtrap Analyst software 1.6.2 and R software version 3.5.1. Statistical analyses were conducted using R software (version 3.5.1; http://www.r-project.org/).

Method: Sixty people age 40-80 were computer randomized into two groups. One lavender top tube was drawn and then spun in Kendro Sorvall centrifuge for 10 minutes at 1300 rcf. The plasma was placed in cryo tubes and flash frozen to -22C then shipped in dry ice to laboratory for analysis. The filtrate was concentrated by speed-vac and reconstituted with de-ionized water to 50 ul and analyzed with AB Sciex API4000 Qtrap. Statistical assessments were evaluated using a nonparametric Wilcoxon signed rank test, p values are two-sided and p<0.05 was used to define statistical significance.

Results: A significant increase in GHK-Cu concentration in the blood of the active group was seen comparing changes from Day 2 to Day 7 between Group A vs. Group B in GHK-Cu Concentration (ng/ml) at p<0.035 and in Total GHK-Cu (ng) at p<0.03.

Conclusion: This study showed a significant increase in the GHK-Cu concentration present in the blood as a result of wearing the LifeWave X39 patch for 1 week in individuals age 40 to 80. This is seen from Day 2 to Day 7 between Active vs. Control in GHK-Cu Concentration (ng/ml) at p<0.035 and in 

Total GHK-Cu (ng) at p<0.03.
Keywords: GHK-Cu, Meridian, Non-transdermal, Photobiology, Phototherapy

This study explores the impact of wearing the LifeWave X39 nontransdermal photobiomodulation patch over the period of one week on levels of glycyl-L-histidyl-L-lysine-copper(2+) (GHK-Cu) levels in the blood in a double-blind randomized controlled trial. This particular tripeptide was first isolated by Dr. Loren Pickart in 1973. GHK-Cu is important as the “copper tripeptide-1 belongs to a group of emergency response molecules which are released during injury and come to the body’s aid...” [1] It is naturally sent by the body to any type of injury to tissue. For example: the “copper tripeptide-1 has been suggested to have a potential therapeutic role in age-related neurodegeneration and cognitive decline. It improves axon survival and maintenance of nerves” [1]. It has been implicated in the resetting of 4000 genes [2]. Blood samples to determine GHK-Cu levels were taken at baseline, 24 hours and at 7 days of wearing the patch. A sample of convenience of 60 subjects made up of both men and women aged 40-81 were selected to participate in this study. Participants were randomized into Group A or Group B by computer.

Melinda H Connor (2021)   Double-Blind Testing of the Lifewave X39 Patch to Determine GHK-Cu Production   Levels

The LifeWave X39 patch uses phototherapy to stimulate a rebalancing of the body. Based on data from other studies, it was felt that a possible change in the copper tripeptide GHK-Cu might be a factor in the effects produced by the patch. As a follow on to prior studies it was determined that a double blind study was an appropriate method of testing this theory. The tripeptide has been demonstrated to improve tissue remodeling in previous research. “It increases keratinocyte proliferation and normal collagen synthesis, improves skin thickness, skin elasticity and firmness, improves wrinkles, photodamage and uneven pigmentation, improves skin clarity, and tightens protective barrier proteins” [3]. Research has identified that the peptide is used to signal the beginning of the natural repair process.

“Copper tripeptide-1(GHK-Cu) is a small protein composed of the three amino acids (protein building blocks) glycine, histidine, and lysine combined in a specific geometric configuration with the physiologically beneficial mineral (copper)” [4]. Later research established the strong affinity between the GHK peptide and copper, and the two forms (GHK and GHK-Cu) it exists in, as this was not covered in the initial experiment. It should also be mentioned that GHK has never been found to cause an issue in all of the research that has been done [1]. 

All X39 patches are sealed to prevent the contact of any of the substances inside to the skin. The sealing of the patches allows for consistent light flow through the patch the entire time that the patch is worn. Patches are designed to reflect wavelengths of light in the infrared, near infrared, and visible light bands. Using the same adhesives as band-aids, this limits the level of irritation which might be developed through consistent daily use of the patch.

Phototherapy has been used for over 100 years in various forms. There has been little evidence of negative side effects throughout that time period. This suggests that phototherapy is a relatively untapped option for healing, and one that has relatively few risks [5].

To determine if the LifeWave X39 non-transdermal photobiomodulation active patch would show improved production of GHK-Cu over controls in a double blind randomized controlled trial.

Once human research studies ethics board approval was received (NFFEH 01-16-20-01) recruitment was begun. Flyers advertising for interested research participants were posted at various local sites. Participants would call into the main study phone number and were assessed for inclusion and exclusion criterion. If appropriate they were scheduled for consenting. At the time of arrival at the study site, each participant was consented and then randomized into group A or B. Individual participants were then taken into the exam room and a blood sample was taken at baseline. Additional samples were taken at 24 hours and 7 days of patch placement.

For convenience, participants were asked to use what is a recognized meridian point, GV14 or CV6 [6], for the patch placement. BD Vacutainer Safety Loc Blood Collection sets were used with Pre-attached holder sized 21GX0.75 or 23GX0.75 and placed in lavender top tubes. Each blood sample was then placed in the Kendro Sorvall Biofuge centrifuge 75005184+ HERAEUS 7591 with a 4000 RPM rotor, spun for 10 minutes at 1300 rcf to separate the plasma, which was then placed in the cryo tubes, and then flash frozen using a medical freezer at -22C. Samples were then placed in 2” thick polystyrene containers, wrapped in thermal box liners and placed in double walled boxes with dry ice for overnight shipping. Samples were sent to HT-Labs, a division of AxisPharm in San Diego, CA.

The blood samples were processed according to the original thesis of Dr. Pickard. The filtrate was concentrated by speed-vac and reconstituted with de-ionized water to 50 ul and analyzed with AB Sciex API4000 Qtrap. The data was analyzed with Analyst software 1.6.2. Values were placed in a spread sheet and then sent for statistical analysis. Both the blood analysis and statistical analysis was done at groups independent from the principle research laboratory.

Absolute changes in GHK and GHK-Cu levels from baseline to the 24 hours and day 7 assessments were summarized in terms of means, standard deviations, medians and ranges. Changes from baseline to the 24 hours and day 7 assessments were evaluated using a nonparametric Wilcoxon signed rank test. All reported p values are two-sided and p<0.05 was used to define statistical significance. Statistical analyses were conducted using R software (version 3.5.1; http://www.r-project.org/). Once the statistical analysis was complete, the blind was broken. Results

A sample of convenience of individuals consisted of 60 individuals randomized into two groups (A and B) with an age range of 41-80. Significant results of the LifeWave X39 patch testing are as follows:

This was a randomized double blind trial which used a sample of convenience recruited from the general population of the greater Tucson, AZ area. Individuals were age 40-81. It should be noted that this trial was interrupted by the COVID SARS-2 pandemic in March of 2020 and resumed in Aug of 2020. At that time special procedures were put in place to be sure of the safety and health of all participants. This included separation of times for scheduled blood draws and special cleaning procedures between each participant: UV-C wanding of all hard surfaces, Clorox wipe of draw chair, changes in gloves and gowns for all study team members and the wearing of masks for both participants and study team members. Study team members were tested weekly to confirm no contagion. No study participant developed COVID SARS-2 through participation in this study process. This study confirmed that there was a significant change in the levels of GHK-Cu in 7 days in both concentration and total amount. This confirms data from earlier studies [7,8]. The repeated trials data supports promotion of positive benefits to the body through increased production of GHK-Cu by the wearing of the LifeWave X39 non-transdermal photobiomodulation patch.

This study explored the changes in amounts of GHK-Cu present in the blood as a result of wearing the LifeWave X39 patch 8-12 hours per day for 1 week. A significant increase in GHK-Cu concentration 

Melinda H   Connor (2021) Double-Blind Testing of the Lifewave X39 Patch to Determine   GHK-Cu Production Levels

Table 1: Study X39 – Descriptive Summary of Outcomes between Groups at Day 1, Day 2, and Day 7.

Table 2: Study X39 - Evaluation of Changes in Outcomes from Day 1 to Day 2, Day 1 to Day 7, and Day 2 to Day 7 within Groups and Comparisons of Changes between Groups.

1p-value for evaluating changes from Day 1 to Day 2, and Day 2 to Day 7 within Control Group. 2p-value for evaluating changes from Day 1 to Day 2, and Day 2 to Day 7 within Active Group. 3p-value for comparing changes from Day 1 to Day 2, and Day 2 to Day 7 between Control Group vs. Active Group.

Citation:

in the blood of the active group was seen in the p-value for comparing changes from Day 2 to Day 7 between Group A vs. Group B in GHK-Cu Concentration (ng/ml) at p<0.035 and in Total GHK-Cu (ng) at p<0.03 (Tables 1 and 2).

1. DeHaven C (2014) Copper Tripeptide-1. Science of Skincare.

2. Pickart L, Vasquez-Soltero J, Margolina A (2014) GHK and DNA: resetting the human genome to health. BioMed Research International2014: 151479. [crossref]

3. Pickart L, Vasquez-Soltero J, Margolina A (2015) GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration. Hindawi Publishing Corporation BioMed Research International 2015: 648108.

4. Geo Peptides Staff (2015) What are Copper Peptides? https://www.geopeptides.com/ copperpep.html

5. Kakimoto C (2017) What is phototherapy, and how does it work? https://www.

dermatologistoncall.com/blog/what-is-phototherapy-and-how-does-it-work/

6. Deadman P, Al-Khafaji M, Baker K (2001) A Manual of Acupuncture. Eastland Press.

7. Connor M, Connor C, Gombosuran N, Eickhoff J, et al. (2020) LifeWave X39 Pilot Demonstrates Light Triggered Changes. International Journal of Healing and Careing www.ijhc.org

8. Connor C, Connor M, Yue D, Chang C, Eickhoff J, et al. (2020) Changes in Tripeptides Produced By the LifeWave X39 Patch. International Journal of Healing and Careing www.ijhc.org